Detailed information NTG0524494

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JGS08_RS01335
• Genome accession: NZ_CP067022
• Coordinates: 284687..285751 (+)
• Length: 354 a.a.
• NCBI ID: WP_007248061.1
• Uniprot ID: A0A3M3ZTK3
• Organism: Pseudomonas cannabina pv. alisalensis strain MAFF 301419
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 279687..290751

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JGS08_RS01325 (JGS08_01325)	-	280578..281846 (+)	1269	WP_007248063.1	malic enzyme-like NAD(P)-binding protein	-
JGS08_RS01330 (JGS08_01330)	-	282033..284477 (-)	2445	WP_371745306.1	penicillin-binding protein 1A	-
JGS08_RS01335 (JGS08_01335)	pilM	284687..285751 (+)	1065	WP_007248061.1	pilus assembly protein PilM	Machinery gene
JGS08_RS01340 (JGS08_01340)	-	285751..286326 (+)	576	WP_007248060.1	PilN domain-containing protein	-
JGS08_RS01345 (JGS08_01345)	pilO	286323..286946 (+)	624	WP_007248059.1	type 4a pilus biogenesis protein PilO	-
JGS08_RS01350 (JGS08_01350)	pilP	286943..287470 (+)	528	WP_007248058.1	type 4a pilus biogenesis lipoprotein PilP	-
JGS08_RS01355 (JGS08_01355)	pilQ	287518..289596 (+)	2079	WP_007248057.1	type IV pilus secretin PilQ	Machinery gene
JGS08_RS01360 (JGS08_01360)	aroK	289601..290119 (+)	519	WP_007248056.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38106.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=524494 JGS08_RS01335 WP_007248061.1 284687..285751(+) (pilM) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=524494 JGS08_RS01335 WP_007248061.1 284687..285751(+) (pilM) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCCTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCTGAACTCGAAGGGGTTGGCCAGGCGTTGTCTCGCGTGCTGGTCAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTT
GCAGTGGCCGGGTCCGCAGTCATCACCAAAACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATTCCCTATCCGCTTGAAGAAGTGGCCATCGATTTTGAAGTGCAGGGCTATTCGGTAC
GCAATCCGGAACGGGTTGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCGGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTTGTTGACGTCGAAGCCTATGCGTTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAATTGGG
GAACGGCCACGACGAGCTGACCGTTGCGGTGGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACCATGGGC
GGATCATCTATACCCGCGAGCAACTGTTCGGCGGCCGCCAGCTGACCGACGAGATTCAGCGCCGCTATGGCCTGTCCATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCTGACGACTATGTCAGCGAGGTGCTGGACCCGTTCAAGGACGC
GCTGGTGCAGCAGGTTTCCCGCTCGTTGCAGTTCTTCTTTGCGGCGGGGCAGTACAACTCGGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCCGGGCTGGAGCACCTGATTCAGCGACGTATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGTTCCAAGGTCAATGCGGGCGCACTGGCCAGTGACGCCCCGGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3M3ZTK3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469