Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH271_RS15875 Genome accession   NZ_CP066982
Coordinates   3601599..3603332 (-) Length   577 a.a.
NCBI ID   WP_227971327.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 27-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3603871..3623217 3601599..3603332 flank 539


Gene organization within MGE regions


Location: 3601599..3623217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH271_RS15875 (JH271_15825) pilB 3601599..3603332 (-) 1734 WP_227971327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH271_RS15880 (JH271_15830) - 3603372..3603782 (-) 411 WP_274316877.1 pilin -
  JH271_RS15885 (JH271_15835) - 3603871..3604287 (-) 417 WP_227971328.1 pilin -
  JH271_RS15890 (JH271_15840) pilC 3604713..3605915 (+) 1203 WP_227971345.1 type II secretion system F family protein Machinery gene
  JH271_RS15895 (JH271_15845) - 3605922..3606785 (+) 864 WP_129588889.1 A24 family peptidase -
  JH271_RS15900 (JH271_15850) coaE 3606799..3607419 (+) 621 WP_129588888.1 dephospho-CoA kinase -
  JH271_RS15905 (JH271_15855) - 3607564..3612324 (+) 4761 WP_274497101.1 RHS repeat-associated core domain-containing protein -
  JH271_RS15910 (JH271_15860) - 3612963..3613390 (+) 428 Protein_3064 SymE family type I addiction module toxin -
  JH271_RS15915 (JH271_15865) - 3613466..3613756 (+) 291 WP_014508712.1 DUF1778 domain-containing protein -
  JH271_RS15920 (JH271_15870) - 3613753..3614244 (+) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH271_RS15925 (JH271_15875) - 3614387..3615721 (-) 1335 WP_162808569.1 HAMP domain-containing sensor histidine kinase -
  JH271_RS15930 (JH271_15880) - 3615714..3616391 (-) 678 WP_003490678.1 response regulator transcription factor -
  JH271_RS15935 (JH271_15885) rimK 3617041..3617916 (-) 876 WP_011038222.1 30S ribosomal protein S6--L-glutamate ligase -
  JH271_RS15940 (JH271_15890) glgX 3618409..3620538 (+) 2130 WP_129588886.1 glycogen debranching protein GlgX -
  JH271_RS15945 (JH271_15895) - 3621073..3621465 (-) 393 WP_129588885.1 H-NS family nucleoid-associated regulatory protein -
  JH271_RS15950 (JH271_15900) - 3621556..3621951 (-) 396 WP_016945003.1 hypothetical protein -
  JH271_RS15955 (JH271_15905) - 3622128..3622283 (-) 156 WP_129588884.1 hypothetical protein -
  JH271_RS15960 (JH271_15910) - 3622347..3622577 (+) 231 WP_165349436.1 hypothetical protein -
  JH271_RS15965 (JH271_15915) - 3622867..3623217 (-) 351 WP_274316879.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62805.97 Da        Isoelectric Point: 5.7640

>NTDB_id=523848 JH271_RS15875 WP_227971327.1 3601599..3603332(-) (pilB) [Xanthomonas campestris pv. campestris strain 27-9]
MNVANSPNLVGITGIARRLVQDGALEEAAARTAMDQATAAKIPLPQWFSEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTQEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=523848 JH271_RS15875 WP_227971327.1 3601599..3603332(-) (pilB) [Xanthomonas campestris pv. campestris strain 27-9]
ATGAACGTGGCTAATTCTCCCAATCTAGTTGGAATTACTGGAATTGCTCGTCGTTTAGTCCAAGACGGAGCGTTGGAAGA
GGCTGCCGCTCGCACCGCAATGGACCAGGCTACGGCAGCCAAAATTCCCTTGCCGCAATGGTTCTCTGAGAAGAAGTTGG
TCACCGCATCCCAGCTCGCTGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGATGGATGTGTCGGTGTTCGATGCCAGT
CAGAATGCAGTCAAGCTGGTGAGTGAGGAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACTAATCTTGTGGTCGAGCCGA
TCCTCGTCGACGAGGATCAGATCCGGCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGTTCGTCGATGGGG
GACGACGACGAGGGGATGGGAGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCACTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGTTGTTGAAGAACGTAGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAACTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGTTACGAGGCAGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCACTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCTTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATTA
CCTCATCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGTAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCAGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGCACCGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

55.106

98.44

0.542
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

49.126

99.133

0.487
  pilF Neisseria gonorrhoeae MS11

48.592

98.44

0.478
  pilB Vibrio campbellii strain DS40M4

46.221

98.614

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.869

96.88

0.454