Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH264_RS15785 Genome accession   NZ_CP066930
Coordinates   3598480..3600213 (-) Length   577 a.a.
NCBI ID   WP_076054161.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 719     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3593480..3605213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH264_RS15775 (JH264_15740) - 3594863..3596473 (+) 1611 WP_076054166.1 HAMP domain-containing sensor histidine kinase -
  JH264_RS15780 (JH264_15745) pilR 3596682..3598076 (+) 1395 WP_042594919.1 sigma-54 dependent transcriptional regulator Regulator
  JH264_RS15785 (JH264_15750) pilB 3598480..3600213 (-) 1734 WP_076054161.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH264_RS15790 (JH264_15755) - 3600317..3600955 (-) 639 WP_076054160.1 methionine biosynthesis protein MetW -
  JH264_RS15795 (JH264_15760) - 3600948..3601994 (-) 1047 WP_081390738.1 glycosyltransferase family 2 protein -
  JH264_RS15800 (JH264_15765) - 3601991..3602785 (-) 795 WP_076057322.1 glycosyltransferase family 2 protein -
  JH264_RS15805 (JH264_15770) - 3602790..3603170 (-) 381 WP_076054155.1 SMR family transporter -
  JH264_RS15810 (JH264_15775) - 3603515..3603931 (+) 417 WP_076054152.1 pilin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62860.94 Da        Isoelectric Point: 5.4486

>NTDB_id=523313 JH264_RS15785 WP_076054161.1 3598480..3600213(-) (pilB) [Xanthomonas campestris pv. raphani strain 719]
MNSVVTANLVGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKRLVTASQLAAANSVEFGMPLLDVSSFDSN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=523313 JH264_RS15785 WP_076054161.1 3598480..3600213(-) (pilB) [Xanthomonas campestris pv. raphani strain 719]
ATGAATAGCGTCGTCACCGCCAACTTGGTTGGCATCACGGGCATTGCCCGGCGGCTTGTGCAGGACGGTGCTCTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCCAAGGTCCCGTTGCCGCAATGGTTCTCAGAGAAGAGGCTGG
TGACCGCTTCGCAACTTGCGGCGGCCAACTCGGTGGAGTTCGGGATGCCGCTGCTTGATGTGTCCTCGTTCGACTCTAAT
CAGAATGCAATCAAATTGGTTAGCGAAGAACTGCTGCAAAAGTTTCAAGTGCTGCCCCTGTTCAAGCGCGGTAATCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCTCTGGACGACATAAAGTTCCATACAAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGATGGGC
GACGACGATGATGAAATGGGGGATCTGGACGTCTCCACTGGGGACGAGGACATGGGCGCCGGTGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACGGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACTGCCGAGGATCCGGTCGAAATCCGGTTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACCGAAGGCTACAAAGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGTGGTAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGTCAGTCGGCATTGATGAAG
GCTTCGCACGGCGTGACTAGCCTGGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.44

0.548
  pilB Acinetobacter baylyi ADP1

55.634

98.44

0.548
  pilB Legionella pneumophila strain ERS1305867

51.408

98.44

0.506
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio cholerae strain A1552

49.365

95.494

0.471
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.648

97.574

0.445