Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   K5T46_RS00065 Genome accession   NZ_CP081489
Coordinates   10784..12277 (-) Length   497 a.a.
NCBI ID   WP_000249329.1    Uniprot ID   -
Organism   Escherichia coli BL21(DE3)     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5784..17277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5T46_RS00035 (K5T46_00035) gspK 6578..7555 (-) 978 WP_000633200.1 type II secretion system minor pseudopilin GspK -
  K5T46_RS00040 (K5T46_00040) gspJ 7552..8157 (-) 606 WP_023147221.1 type II secretion system minor pseudopilin GspJ -
  K5T46_RS00045 (K5T46_00045) gspI 8154..8525 (-) 372 WP_000820109.1 type II secretion system minor pseudopilin GspI -
  K5T46_RS00050 (K5T46_00050) gspH 8522..9085 (-) 564 WP_001115147.1 type II secretion system minor pseudopilin GspH -
  K5T46_RS00055 (K5T46_00055) gspG 9089..9544 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  K5T46_RS00060 (K5T46_00060) gspF 9561..10784 (-) 1224 WP_001173416.1 type II secretion system inner membrane protein GspF -
  K5T46_RS00065 (K5T46_00065) pilB/pilB1 10784..12277 (-) 1494 WP_000249329.1 type II secretion system ATPase GspE Machinery gene
  K5T46_RS00070 (K5T46_00070) gspD 12277..14337 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  K5T46_RS00075 (K5T46_00075) gspC 14367..15326 (-) 960 WP_014639304.1 type II secretion system protein GspC -
  K5T46_RS00080 (K5T46_00080) gspS2 15344..15754 (-) 411 WP_001305972.1 type II secretion system pilot lipoprotein GspS-beta -
  K5T46_RS00085 (K5T46_00085) pilD 15820..16629 (-) 810 WP_000895873.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55230.39 Da        Isoelectric Point: 6.0089

>NTDB_id=522706 K5T46_RS00065 WP_000249329.1 10784..12277(-) (pilB/pilB1) [Escherichia coli BL21(DE3)]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQALPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=522706 K5T46_RS00065 WP_000249329.1 10784..12277(-) (pilB/pilB1) [Escherichia coli BL21(DE3)]
ATGGTGCCTGTAGCACAGGAAACCACTGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGTCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTAGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATTACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGTACGCTGTGCCCCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362