Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   JG532_RS25505 Genome accession   NZ_CP066748
Coordinates   5094331..5094867 (-) Length   178 a.a.
NCBI ID   WP_000168305.1    Uniprot ID   A0A9P2PPK2
Organism   Escherichia coli O157:H7 strain ECP19-2498     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 5094331..5132935 5094331..5094867 within 0


Gene organization within MGE regions


Location: 5094331..5132935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG532_RS25505 (JG532_25505) ssb 5094331..5094867 (-) 537 WP_000168305.1 single-stranded DNA-binding protein SSB1 Machinery gene
  JG532_RS25510 (JG532_25510) uvrA 5095122..5097944 (+) 2823 WP_000357763.1 excinuclease ABC subunit UvrA -
  JG532_RS25515 (JG532_25515) yjbR 5097979..5098335 (-) 357 WP_000155657.1 MmcQ/YjbR family DNA-binding protein -
  JG532_RS25520 (JG532_25520) yjbQ 5098339..5098755 (-) 417 WP_000270375.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  JG532_RS25525 (JG532_25525) aphA 5098866..5099579 (-) 714 WP_001226923.1 acid phosphatase AphA -
  JG532_RS25530 (JG532_25530) - 5099980..5100339 (+) 360 WP_001303754.1 hypothetical protein -
  JG532_RS25535 (JG532_25535) tyrB 5100678..5101871 (-) 1194 WP_000486982.1 aromatic amino acid transaminase -
  JG532_RS25540 (JG532_25540) alr 5102124..5103203 (-) 1080 WP_001147332.1 alanine racemase -
  JG532_RS25545 (JG532_25545) dnaB 5103256..5104671 (-) 1416 WP_000918366.1 replicative DNA helicase -
  JG532_RS25550 (JG532_25550) qorA 5104754..5105737 (+) 984 WP_000235522.1 quinone oxidoreductase -
  JG532_RS25555 (JG532_25555) pspG 5105903..5106145 (-) 243 WP_000891404.1 envelope stress response protein PspG -
  JG532_RS25560 (JG532_25560) dusA 5106279..5107316 (-) 1038 WP_000543819.1 tRNA dihydrouridine(20/20a) synthase DusA -
  JG532_RS25565 (JG532_25565) - 5107405..5108502 (+) 1098 WP_000332269.1 site-specific integrase -
  JG532_RS25570 (JG532_25570) - 5108564..5108812 (+) 249 WP_001217541.1 DinI-like family protein -
  JG532_RS29365 - 5108823..5108942 (-) 120 Protein_5081 capsid protein -
  JG532_RS25575 (JG532_25575) - 5108973..5109614 (-) 642 WP_001143816.1 DUF1076 domain-containing protein -
  JG532_RS25580 (JG532_25580) - 5109696..5110325 (-) 630 WP_072140863.1 DUF1076 domain-containing protein -
  JG532_RS25585 (JG532_25585) - 5110398..5110970 (-) 573 WP_032311934.1 DUF1076 domain-containing protein -
  JG532_RS25590 (JG532_25590) - 5111003..5111080 (-) 78 Protein_5085 tail assembly protein -
  JG532_RS25595 (JG532_25595) - 5111082..5111351 (-) 270 WP_001023355.1 phage tail fiber C-terminal domain-containing protein -
  JG532_RS25600 (JG532_25600) - 5111353..5112666 (-) 1314 WP_000268945.1 prophage tail fiber N-terminal domain-containing protein -
  JG532_RS25605 (JG532_25605) - 5112731..5113330 (-) 600 WP_001230514.1 Ail/Lom family outer membrane beta-barrel protein -
  JG532_RS25610 (JG532_25610) - 5113398..5114120 (-) 723 Protein_5089 DUF1983 domain-containing protein -
  JG532_RS25615 (JG532_25615) - 5114108..5114524 (+) 417 Protein_5090 DUF2303 family protein -
  JG532_RS25620 (JG532_25620) yfdR 5114652..5115188 (+) 537 WP_000008211.1 5'-deoxynucleotidase -
  JG532_RS25625 (JG532_25625) - 5115179..5115529 (+) 351 WP_001242740.1 hypothetical protein -
  JG532_RS29070 - 5115526..5115999 (+) 474 Protein_5093 SAM-dependent methyltransferase -
  JG532_RS25635 (JG532_25635) - 5116146..5116343 (+) 198 WP_000829415.1 hypothetical protein -
  JG532_RS25640 (JG532_25640) - 5116301..5116651 (+) 351 Protein_5095 3'-5' exonuclease -
  JG532_RS25645 (JG532_25645) - 5116688..5116969 (+) 282 WP_001093918.1 pyocin activator PrtN family protein -
  JG532_RS25650 (JG532_25650) - 5117017..5117190 (-) 174 WP_000654815.1 hypothetical protein -
  JG532_RS25655 (JG532_25655) - 5117387..5117920 (-) 534 WP_000956557.1 hypothetical protein -
  JG532_RS25660 (JG532_25660) - 5118067..5118171 (-) 105 Protein_5099 tRNA-dihydrouridine synthase -
  JG532_RS25665 (JG532_25665) - 5118533..5119489 (-) 957 WP_000912588.1 DUF2713 family protein -
  JG532_RS25670 (JG532_25670) zur 5119808..5120323 (+) 516 WP_001301681.1 zinc uptake transcriptional repressor Zur -
  JG532_RS25675 (JG532_25675) yjbJ 5120365..5120574 (-) 210 WP_001030593.1 CsbD family protein -
  JG532_RS25680 (JG532_25680) dinF 5120690..5122015 (-) 1326 WP_001301558.1 MATE family efflux transporter DinF -
  JG532_RS25685 (JG532_25685) lexA 5122088..5122696 (-) 609 WP_000646078.1 transcriptional repressor LexA -
  JG532_RS25690 (JG532_25690) dgkA 5122806..5123174 (-) 369 WP_000002907.1 diacylglycerol kinase -
  JG532_RS25695 (JG532_25695) plsB 5123345..5125768 (+) 2424 WP_001301502.1 glycerol-3-phosphate 1-O-acyltransferase PlsB -
  JG532_RS25700 (JG532_25700) ubiA 5125923..5126795 (-) 873 WP_000455227.1 4-hydroxybenzoate octaprenyltransferase -
  JG532_RS25705 (JG532_25705) ubiC 5126808..5127305 (-) 498 WP_001301929.1 chorismate lyase -
  JG532_RS25710 (JG532_25710) - 5127528..5129108 (-) 1581 WP_000900547.1 SopA family protein -
  JG532_RS25715 (JG532_25715) malM 5129337..5130257 (-) 921 WP_001301777.1 maltose operon protein MalM -
  JG532_RS25720 (JG532_25720) lamB 5130408..5131748 (-) 1341 WP_000973673.1 maltoporin LamB -
  JG532_RS25725 (JG532_25725) malK 5131820..5132935 (-) 1116 WP_000179165.1 maltose/maltodextrin ABC transporter ATP-binding protein MalK -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18975.00 Da        Isoelectric Point: 5.2358

>NTDB_id=522343 JG532_RS25505 WP_000168305.1 5094331..5094867(-) (ssb) [Escherichia coli O157:H7 strain ECP19-2498]
MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYI
EGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
PAAPSNEPPMDFDDDIPF

Nucleotide


Download         Length: 537 bp        

>NTDB_id=522343 JG532_RS25505 WP_000168305.1 5094331..5094867(-) (ssb) [Escherichia coli O157:H7 strain ECP19-2498]
ATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGG
TGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAGCAGACTG
AATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCCAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATC
GAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTACACCACAGAAGTCGTGGTGAACGTTGG
CGGCACCATGCAGATGCTGGGTGGTCGTCAGGGTGGTGGCGCTCCTGCAGGTGGCAATATCGGTGGTGGTCAGCCGCAGG
GCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCT
CCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGACGATATTCCGTTCTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(6-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

74.444

100

0.753
  ssb Glaesserella parasuis strain SC1401

57.923

100

0.596
  ssb Neisseria meningitidis MC58

48.066

100

0.489
  ssb Neisseria gonorrhoeae MS11

48.066

100

0.489