NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	I5P57_RS02170	Genome accession	NZ_CP066740
Coordinates	450278..451402 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli strain R12
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 445278..456402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5P57_RS02145 (I5P57_00427)	mscL	445401..445814 (-)	414	WP_000022445.1	large-conductance mechanosensitive channel protein MscL	-
I5P57_RS02150 (I5P57_00428)	trkA	445944..447320 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
I5P57_RS02155 (I5P57_00429)	rsmB	447342..448631 (-)	1290	WP_001765229.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
I5P57_RS02160 (I5P57_00430)	fmt	448677..449624 (-)	948	WP_001765230.1	methionyl-tRNA formyltransferase	-
I5P57_RS02165 (I5P57_00431)	def	449639..450148 (-)	510	WP_000114984.1	peptide deformylase	-
I5P57_RS02170 (I5P57_00432)	dprA	450278..451402 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
I5P57_RS02175 (I5P57_00433)	smg	451374..451847 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
I5P57_RS02180 (I5P57_00434)	yrdD	451876..452418 (+)	543	WP_001312137.1	type I DNA topoisomerase	-
I5P57_RS02185 (I5P57_00435)	tsaC	452423..452995 (+)	573	WP_001765231.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
I5P57_RS02190 (I5P57_00436)	aroE	453000..453818 (+)	819	WP_001765233.1	shikimate dehydrogenase	-
I5P57_RS02195 (I5P57_00437)	yrdB	453815..454072 (+)	258	WP_001765234.1	DUF1488 domain-containing protein	-
I5P57_RS02200 (I5P57_00438)	yrdA	454048..454602 (-)	555	WP_001525792.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=522217 I5P57_RS02170 WP_000228517.1 450278..451402(+) (dprA) [Escherichia coli strain R12]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=522217 I5P57_RS02170 WP_000228517.1 450278..451402(+) (dprA) [Escherichia coli strain R12]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0522217