Detailed information NTG0052216

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: P423_RS10270
• Genome accession: NC_022648
• Coordinates: 2002472..2003482 (-)
• Length: 336 a.a.
• NCBI ID: WP_000568520.1
• Uniprot ID: -
• Organism: Escherichia coli JJ1886
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1997472..2008482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P423_RS10240 (P423_09855)	znuA	1998272..1999204 (-)	933	WP_001347089.1	zinc ABC transporter substrate-binding protein ZnuA	-
P423_RS10245 (P423_09860)	znuC	1999283..2000038 (+)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
P423_RS10250 (P423_09865)	znuB	2000035..2000820 (+)	786	WP_000571478.1	zinc ABC transporter permease subunit ZnuB	-
P423_RS10270 (P423_09885)	ruvB	2002472..2003482 (-)	1011	WP_000568520.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
P423_RS10275 (P423_09890)	ruvA	2003491..2004102 (-)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
P423_RS29290	yobI	2004241..2004306 (-)	66	WP_072093883.1	stress response small protein YobI	-
P423_RS10280 (P423_09895)	yebB	2004377..2004979 (+)	603	WP_001024911.1	YebB family permuted papain-like enzyme	-
P423_RS10285 (P423_09900)	ruvC	2004981..2005502 (-)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
P423_RS10290 (P423_09905)	yebC	2005537..2006277 (-)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
P423_RS10295 (P423_09910)	nudB	2006306..2006758 (-)	453	WP_077249130.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37163.73 Da
• Isoelectric Point: 4.7818

>NTDB_id=52216 P423_RS10270 WP_000568520.1 2002472..2003482(-) (ruvB) [Escherichia coli JJ1886]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMS

Nucleotide

• Length: 1011 bp

>NTDB_id=52216 P423_RS10270 WP_000568520.1 2002472..2003482(-) (ruvB) [Escherichia coli JJ1886]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGCGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGTCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494