Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC/celB   Type   Machinery gene
Locus tag   IGS65_RS04865 Genome accession   NZ_CP066294
Coordinates   994831..997065 (-) Length   744 a.a.
NCBI ID   WP_002261915.1    Uniprot ID   A0AAX1K0M7
Organism   Streptococcus mutans strain 27-3     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 989831..1002065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGS65_RS04845 (IGS65_004845) - 990437..991501 (-) 1065 WP_019803953.1 serine hydrolase -
  IGS65_RS04850 (IGS65_004850) sodA 992053..992664 (-) 612 WP_002261912.1 superoxide dismutase SodA -
  IGS65_RS04855 (IGS65_004855) holA 992811..993851 (-) 1041 WP_002286210.1 DNA polymerase III subunit delta -
  IGS65_RS04860 (IGS65_004860) - 994019..994645 (-) 627 WP_002264731.1 DUF805 domain-containing protein -
  IGS65_RS04865 (IGS65_004865) comEC/celB 994831..997065 (-) 2235 WP_002261915.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IGS65_RS04870 (IGS65_004870) - 997055..997735 (-) 681 WP_002270597.1 helix-hairpin-helix domain-containing protein -
  IGS65_RS04875 (IGS65_004875) - 997875..998627 (-) 753 WP_002267103.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  IGS65_RS04880 (IGS65_004880) - 998766..999701 (+) 936 WP_002278209.1 polysaccharide deacetylase family protein -
  IGS65_RS04885 (IGS65_004885) - 999790..1000302 (+) 513 WP_002261919.1 HXXEE domain-containing protein -
  IGS65_RS04890 (IGS65_004890) - 1000348..1001367 (+) 1020 WP_002280666.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 744 a.a.        Molecular weight: 85545.98 Da        Isoelectric Point: 10.0891

>NTDB_id=519124 IGS65_RS04865 WP_002261915.1 994831..997065(-) (comEC/celB) [Streptococcus mutans strain 27-3]
MLIRFFPLKPIYLAFLTVLLYFCIHCFSFLPVGLFILALVFLVFQYDRQICIKTLLFLLPFAAFFFYFHQKRQSEYQIVP
RQVQKLIIIPDTLSVNGDNLSFRAKSGHWTYQVFYKLKSETEKHYFEQLWQTAEIQVTADVVEAEEQRNFKGFDYRNYLK
MQGIYRIVNVTDIKTIKLSSKRFWFARLHEWRRRALVSIQSKFPDPMKHYMTGLLFGYLDKSFDEMNAIYSSLGIIHLFA
LSGMQVSFFIGKFRYLGLRLGIRQEYMNAIQLPFSLLYAGLTGFAVSVVRSLIQGNLTHFGFKKQDNFALTLFVMFFLIP
NFLLTTGGVLSFAYAFILIMIDFETLSLLKRILFQTFSLSLGILPLLMWYFSSFQPLSILLTILFSFIFDNLMLPFLTLA
YFLSPFVSLACFNPAFRLLERIIVQIHLIFSRPFILGSPTLPILLLLLCLLALLYDFRQKKRVVLVLSSILALLFFISKN
PLTNEVTIVDIGQGDSILLRDISSKTILIDVGGKVTFGQTDKWRRRISDTNAEKTLIPYLKSRGIDKIDQLVLTHTDTDH
MGDMEVVAKAFHVGEVLVSPGSLTKANFVSKLRRMKVKVHITKPGETLSIMGSHLQVLYPLKIGDGGNNDSIVLYGRLLG
LNFLFTGDLEKEGEAELLKTYPNLKVDVLKAGHHGSKGSSSPELLAQIEPKIALISAGKNNRYKHPHQETLDRFAQIKSR
IYRTDQEGAIRFKGLKHWQIETVR

Nucleotide


Download         Length: 2235 bp        

>NTDB_id=519124 IGS65_RS04865 WP_002261915.1 994831..997065(-) (comEC/celB) [Streptococcus mutans strain 27-3]
GTGTTGATTAGGTTTTTTCCTCTCAAACCAATCTATTTAGCCTTTTTAACTGTTTTGCTTTATTTTTGTATTCACTGCTT
TTCTTTTTTGCCAGTTGGCCTATTTATTTTAGCTTTAGTCTTTCTTGTTTTTCAATATGATAGGCAAATCTGTATCAAAA
CACTTTTGTTTTTATTGCCTTTTGCCGCTTTCTTTTTCTATTTTCACCAAAAAAGGCAAAGCGAGTATCAAATAGTACCC
AGACAGGTTCAAAAGTTAATTATCATTCCAGATACGCTTAGTGTCAATGGAGATAACTTAAGTTTTCGAGCAAAATCAGG
TCATTGGACTTATCAGGTCTTTTATAAATTAAAATCTGAAACAGAGAAACATTATTTTGAACAATTATGGCAGACAGCAG
AAATTCAAGTGACAGCAGATGTTGTAGAAGCAGAAGAGCAGCGTAATTTCAAGGGATTCGATTATAGAAACTATTTAAAG
ATGCAGGGAATTTACCGAATTGTTAATGTTACAGACATTAAAACAATTAAACTGAGTTCTAAACGCTTCTGGTTTGCTCG
TTTGCATGAATGGCGACGAAGAGCTTTGGTATCTATTCAGTCAAAATTTCCAGACCCCATGAAACACTATATGACTGGTC
TGCTTTTTGGCTATTTAGATAAATCCTTTGATGAAATGAATGCTATTTATTCTAGCTTAGGTATTATTCACCTTTTTGCT
TTGTCAGGTATGCAGGTTAGTTTTTTCATTGGGAAATTTCGTTATTTGGGCTTGCGTTTAGGTATTAGACAGGAATACAT
GAATGCTATTCAGCTGCCCTTTTCTTTGCTTTATGCTGGTTTGACTGGCTTTGCAGTATCCGTTGTGCGTAGTTTGATTC
AAGGTAATCTTACTCATTTTGGTTTTAAAAAGCAGGATAACTTTGCTCTAACCTTATTTGTTATGTTTTTCCTGATACCC
AATTTCTTATTAACAACAGGAGGTGTGCTTAGTTTTGCCTATGCTTTTATTCTAATCATGATTGATTTTGAGACTTTGTC
ACTCTTAAAGCGGATTCTATTTCAAACCTTTTCTCTTTCTTTAGGGATTTTACCGTTATTGATGTGGTATTTTAGCAGTT
TTCAGCCCTTGTCTATCCTATTAACTATCCTCTTTTCTTTTATTTTTGATAACTTGATGTTGCCTTTTTTGACACTTGCT
TATTTTCTGTCACCTTTTGTATCACTTGCCTGCTTCAATCCAGCTTTTAGATTATTAGAAAGAATAATTGTTCAAATTCA
CCTAATCTTTAGCCGTCCTTTTATTTTGGGCAGTCCGACTTTGCCTATTCTCTTACTTTTATTGTGCCTTTTAGCTTTAC
TCTATGATTTTCGTCAGAAAAAAAGGGTAGTGTTAGTACTTAGTAGCATTCTCGCCCTGTTATTTTTCATCAGTAAAAAT
CCTTTGACTAATGAAGTGACTATTGTTGATATTGGTCAGGGGGATAGTATTCTTCTTCGGGATATCAGCAGTAAGACTAT
TTTAATTGATGTAGGAGGAAAAGTAACCTTTGGACAGACTGATAAATGGCGGCGGCGTATCAGTGATACTAATGCTGAAA
AGACTTTGATTCCCTATCTCAAAAGCAGAGGCATTGATAAGATTGACCAATTGGTTTTGACTCATACTGACACGGATCAT
ATGGGAGATATGGAAGTGGTGGCAAAGGCCTTTCATGTTGGGGAAGTTTTAGTTAGTCCAGGCAGTTTAACTAAGGCAAA
TTTTGTCAGTAAATTGAGAAGAATGAAAGTTAAGGTACACATCACTAAGCCAGGGGAGACATTATCCATTATGGGGAGTC
ATTTACAGGTACTCTATCCGTTGAAAATAGGTGATGGTGGTAATAATGACTCTATCGTTCTTTATGGACGTTTGCTAGGC
TTAAATTTTCTCTTTACAGGAGATTTGGAAAAAGAAGGAGAAGCAGAATTGCTAAAGACTTATCCCAATTTAAAAGTTGA
TGTCTTAAAAGCTGGACATCATGGTTCAAAAGGTTCTTCTTCACCTGAATTATTAGCTCAAATAGAGCCTAAAATTGCTC
TTATCTCGGCAGGAAAGAACAATCGTTATAAACATCCCCATCAGGAGACACTGGACAGATTTGCGCAAATAAAAAGTCGG
ATTTATCGGACGGATCAAGAGGGTGCCATTCGTTTTAAGGGTCTTAAGCATTGGCAGATTGAGACTGTGCGATAA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(30-174)

Competence protein (Competence)

(214-460)

Metallo-beta-lactamase superfamily (Lactamase_B)

(492-698)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus mitis NCTC 12261

51.213

99.731

0.511
  comEC/celB Streptococcus mitis SK321

51.282

99.597

0.511
  comEC/celB Streptococcus pneumoniae TIGR4

50.806

100

0.508
  comEC/celB Streptococcus pneumoniae Rx1

50.134

100

0.501
  comEC/celB Streptococcus pneumoniae D39

50.134

100

0.501
  comEC/celB Streptococcus pneumoniae R6

50.134

100

0.501
  comEC Lactococcus lactis subsp. cremoris KW2

46.309

100

0.464