Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I9H06_RS23245 Genome accession   NZ_CP066267
Coordinates   5123486..5124550 (-) Length   354 a.a.
NCBI ID   WP_005888204.1    Uniprot ID   A0AA40P5K0
Organism   Pseudomonas tremae strain PA-1-12B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5118486..5129550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H06_RS23220 (I9H06_23110) aroK 5119091..5119609 (-) 519 WP_005888214.1 shikimate kinase AroK -
  I9H06_RS23225 (I9H06_23115) pilQ 5119614..5121719 (-) 2106 WP_005888212.1 type IV pilus secretin PilQ Machinery gene
  I9H06_RS23230 (I9H06_23120) pilP 5121767..5122294 (-) 528 WP_005888210.1 type 4a pilus biogenesis lipoprotein PilP -
  I9H06_RS23235 (I9H06_23125) pilO 5122291..5122914 (-) 624 WP_005888208.1 type 4a pilus biogenesis protein PilO -
  I9H06_RS23240 (I9H06_23130) - 5122911..5123486 (-) 576 WP_005888206.1 PilN domain-containing protein -
  I9H06_RS23245 (I9H06_23135) pilM 5123486..5124550 (-) 1065 WP_005888204.1 pilus assembly protein PilM Machinery gene
  I9H06_RS23250 (I9H06_23140) - 5124758..5127202 (+) 2445 WP_005888202.1 penicillin-binding protein 1A -
  I9H06_RS23255 (I9H06_23145) - 5127365..5128633 (-) 1269 WP_005888200.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38103.67 Da        Isoelectric Point: 4.7688

>NTDB_id=519042 I9H06_RS23245 WP_005888204.1 5123486..5124550(-) (pilM) [Pseudomonas tremae strain PA-1-12B]
MFELFKKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=519042 I9H06_RS23245 WP_005888204.1 5123486..5124550(-) (pilM) [Pseudomonas tremae strain PA-1-12B]
GTGTTCGAACTCTTCAAGAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTGGTCGAAAAAAATA
TTGCCGAACTCGAAGGGGTGGGTCAGGCACTGTCTCGCGTGCTTGCCAAGGCAAAGACCAGCGTCAAGATTGTGGCGGTG
GCCGTTGCCGGGTCCGCCGTTATTACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGATCAGTACATTCCCTACCCGCTTGAAGAAGTCGCCATCGATTTTGAGGTGCAGGGCTATTCGGTCC
GCAACCCGGAACGCGTCGAGGTCCTTCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCGCTG
GCCGGTCTGACCGCGCGTGTCGTCGATGTCGAAGCTTATGCGCTGGAGCGTTCGTTCGGCCTGCTGGCCGCGCAACTGGG
CAACGGTCATGACGAGCTTACCGTGGCGGTGGTTGATATCGGCGCCACAATGACCACCTTGAGCGTGCTGCACCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTCGGTGGTCGCCAGCTGACTGACGAAATTCAGCGCCGTTACGGGCTCTCGATG
GAGGAAGCCGGGCTTGCCAAAAAGCAGGGCGGGCTGCCCGACGACTACGTCAGCGAAGTGCTGGACCCGTTCAAGGATGC
ATTGGTGCAGCAGGTTTCCCGTTCTCTGCAGTTCTTTTTTGCGGCAGGTCAGTACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCAATCTCCGGGCTGGAGCATCTGATCCAGCGGCGCATTGGTACGCCGACTATGGTGGCCAACCCG
TTTGCCGACATGGCTTTGAGCGCCAAGGTCAATGCTGGCGCCCTGGCGAGTGATGCGCCTGCCTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472