Detailed information NTG0516746

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: I6I22_RS05130
• Genome accession: NZ_CP066045
• Coordinates: 1098253..1099317 (+)
• Length: 354 a.a.
• NCBI ID: WP_023446117.1
• Uniprot ID: V4QEI0
• Organism: Stutzerimonas stutzeri strain FDAARGOS_1065
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1093253..1104317

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6I22_RS05115 (I6I22_05110)	-	1093364..1094158 (+)	795	WP_101276068.1	thermonuclease family protein	-
I6I22_RS05120 (I6I22_05115)	-	1094308..1095576 (+)	1269	WP_014821724.1	malic enzyme-like NAD(P)-binding protein	-
I6I22_RS05125 (I6I22_05120)	-	1095640..1098072 (-)	2433	WP_101276066.1	penicillin-binding protein 1A	-
I6I22_RS05130 (I6I22_05125)	pilM	1098253..1099317 (+)	1065	WP_023446117.1	pilus assembly protein PilM	Machinery gene
I6I22_RS05135 (I6I22_05130)	pilN	1099317..1099889 (+)	573	WP_014821721.1	type 4a pilus biogenesis protein PilN	-
I6I22_RS05140 (I6I22_05135)	pilO	1099886..1100509 (+)	624	Protein_1022	type 4a pilus biogenesis protein PilO	-
I6I22_RS05145 (I6I22_05140)	pilP	1100506..1101033 (+)	528	WP_101276062.1	type 4a pilus biogenesis lipoprotein PilP	-
I6I22_RS05150 (I6I22_05145)	pilQ	1101087..1103174 (+)	2088	WP_101276060.1	type IV pilus secretin PilQ	Machinery gene
I6I22_RS05155 (I6I22_05150)	aroK	1103180..1103725 (+)	546	WP_014821719.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37671.17 Da
• Isoelectric Point: 4.6880

>NTDB_id=516746 I6I22_RS05130 WP_023446117.1 1098253..1099317(+) (pilM) [Stutzerimonas stutzeri strain FDAARGOS_1065]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYRVEAYAVEPLPANAVVEKNIAELEGVGQALQRVVAKAKTGCKSAVV
AVSGSAVITKIIEMDAGLSDEELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRSPGRAEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYGLLAPQLGAAHGELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSLQFFFAAGQFHDVDCILLAGGTASIPGLDHMIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=516746 I6I22_RS05130 WP_023446117.1 1098253..1099317(+) (pilM) [Stutzerimonas stutzeri strain FDAARGOS_1065]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACGCTGCTTGGGATCGATATCAGTTCGACCTCAGTCAAGCTCCTCGAATT
AAGTCGATCAGGTAGTCGCTACCGCGTCGAGGCGTATGCTGTCGAGCCGCTCCCGGCAAACGCCGTAGTTGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCATTGCAGCGGGTGGTTGCCAAGGCCAAGACCGGATGCAAGTCGGCGGTAGTG
GCTGTTTCAGGTTCCGCGGTAATCACCAAGATCATCGAGATGGATGCTGGTTTGTCCGACGAAGAGCTCGAGAATCAGCT
GAAGATCGAGGCGGACCAGTACATTCCCTATCCGCTGGAAGAAGTGGCGATCGACTTCGAAGTCCAGGGGCCTGCTCCTC
GCTCTCCTGGCCGAGCAGAAGTCCTGCTCGCCGCTTGCCGCAAGGAAAACGTCGAAATTCGTGAGGCAGCGCTTGCGCTG
GCGGGGCTCACTGCCAAGGTGGTTGACGTCGAAGCCTATGCGCTCGAGCGGTCTTACGGGCTACTCGCTCCACAGTTGGG
TGCGGCGCATGGAGAGCTGACCGTTGCCGTGGTGGATATCGGTGCCACCATGACTACCCTCAGTGTTCTGCATAACGGTC
GTACTATTTATACCCGTGAACAGCTTTTCGGCGGGCGTCAGCTGACGGAGGAAATTCAGCGACGCTACGGCCTCTCACAA
GAAGAAGCTGGTCTAGCCAAAAAGCAAGGCGGCCTCCCAGACGACTACGACAGTGAGGTTTTGCTTCCGTTCAAAGAGGC
AGTTGTGCAGCAGGTTTCTCGCTCGTTGCAGTTCTTCTTTGCGGCAGGACAGTTCCATGATGTCGACTGCATCCTGTTAG
CGGGCGGGACGGCATCGATACCTGGGTTGGATCACATGATTCAGCAGAAGATCGGTACTCAGACTCTAGTTGCGAATCCA
TTCGCCGATATGGCTTTGAGCAGCAAAGTGAATGCTGGAGCGCTGGCTAGCGATGCGCCGGCCTTGATGATTGCTTGTGG
CCTGGCGCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: V4QEI0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	53.107	100	0.531
pilM	Legionella pneumophila strain ERS1305867	47.175	100	0.472