Detailed information NTG0516738

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6I22_RS00525
• Genome accession: NZ_CP066045
• Coordinates: 119629..120774 (-)
• Length: 381 a.a.
• NCBI ID: WP_014818747.1
• Uniprot ID: I4CN99
• Organism: Stutzerimonas stutzeri strain FDAARGOS_1065
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 114629..125774

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6I22_RS00500 (I6I22_00500)	-	114688..115704 (+)	1017	WP_101275336.1	aspartate carbamoyltransferase catalytic subunit	-
I6I22_RS00505 (I6I22_00505)	-	115704..116978 (+)	1275	WP_023446074.1	dihydroorotase	-
I6I22_RS00510 (I6I22_00510)	-	117056..117451 (-)	396	WP_003282737.1	NINE protein	-
I6I22_RS00515 (I6I22_00515)	-	117585..118796 (+)	1212	WP_041015122.1	putative DNA modification/repair radical SAM protein	-
I6I22_RS00520 (I6I22_00520)	-	118796..119632 (+)	837	WP_101275337.1	TIGR03915 family putative DNA repair protein	-
I6I22_RS00525 (I6I22_00525)	pilU	119629..120774 (-)	1146	WP_014818747.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6I22_RS00530 (I6I22_00530)	pilT	120835..121869 (-)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
I6I22_RS00535 (I6I22_00535)	-	121934..122623 (+)	690	WP_101275338.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6I22_RS00540 (I6I22_00540)	proC	122634..123458 (+)	825	WP_101275339.1	pyrroline-5-carboxylate reductase	-
I6I22_RS00545 (I6I22_00545)	-	123509..124099 (+)	591	WP_041015113.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42457.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=516738 I6I22_RS00525 WP_014818747.1 119629..120774(-) (pilU) [Stutzerimonas stutzeri strain FDAARGOS_1065]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=516738 I6I22_RS00525 WP_014818747.1 119629..120774(-) (pilU) [Stutzerimonas stutzeri strain FDAARGOS_1065]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TTATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAATCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGGGCCACCGGTA
CCGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
GGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTAATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGTCACCTCTGCTTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGCCCGACCGACACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGGCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGTGAAATTACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGATTTGCGTCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGACCAGCGTGTCCCAAGGCCTGAGCCTAGAGGTCAGTGACG
ACGATCCAGGACGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: I4CN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388