Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   THIVI_RS13840 Genome accession   NC_018012
Coordinates   3135373..3136464 (+) Length   363 a.a.
NCBI ID   WP_014779196.1    Uniprot ID   I3YCP6
Organism   Thiocystis violascens DSM 198     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3111642..3140645 3135373..3136464 within 0


Gene organization within MGE regions


Location: 3111642..3140645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIVI_RS13760 (Thivi_2834) - 3111642..3113018 (-) 1377 WP_014779183.1 hypothetical protein -
  THIVI_RS25800 (Thivi_2835) - 3113195..3113686 (+) 492 WP_052315030.1 IS5 family transposase -
  THIVI_RS25805 (Thivi_2836) - 3113795..3113998 (+) 204 WP_245537274.1 transposase -
  THIVI_RS24740 - 3114189..3114515 (-) 327 WP_157174452.1 hypothetical protein -
  THIVI_RS24745 - 3114719..3115120 (-) 402 WP_157174453.1 hypothetical protein -
  THIVI_RS24750 - 3115471..3115872 (-) 402 WP_157174454.1 hypothetical protein -
  THIVI_RS13770 (Thivi_2837) - 3116021..3117217 (-) 1197 WP_014779184.1 FAD-dependent oxidoreductase -
  THIVI_RS13775 (Thivi_2838) - 3117227..3117748 (-) 522 WP_014779185.1 hypothetical protein -
  THIVI_RS13780 (Thivi_2839) - 3117794..3118393 (-) 600 WP_014779186.1 DUF3365 domain-containing protein -
  THIVI_RS13785 (Thivi_2840) - 3118527..3122066 (-) 3540 WP_014779187.1 EAL domain-containing protein -
  THIVI_RS13790 (Thivi_2842) dnaN 3122353..3123453 (-) 1101 WP_014779188.1 DNA polymerase III subunit beta -
  THIVI_RS23785 (Thivi_2843) dnaA 3123530..3124867 (-) 1338 WP_014779189.1 chromosomal replication initiator protein DnaA -
  THIVI_RS13800 (Thivi_2844) - 3125132..3126241 (+) 1110 WP_041447215.1 IS5 family transposase -
  THIVI_RS13805 (Thivi_2845) uvrD 3126846..3129017 (+) 2172 WP_014779190.1 DNA helicase II -
  THIVI_RS13810 (Thivi_2846) rpsA 3129101..3130315 (+) 1215 WP_014779191.1 30S ribosomal protein S1 -
  THIVI_RS13815 (Thivi_2847) hemW 3130334..3131548 (+) 1215 WP_014779192.1 radical SAM family heme chaperone HemW -
  THIVI_RS13820 rpmE 3131743..3131943 (+) 201 WP_041447608.1 50S ribosomal protein L31 -
  THIVI_RS13825 (Thivi_2849) - 3132318..3132819 (+) 502 Protein_2817 hypothetical protein -
  THIVI_RS13830 (Thivi_2850) - 3133003..3134298 (+) 1296 WP_014779194.1 citrate synthase -
  THIVI_RS13835 (Thivi_2851) - 3134295..3134909 (-) 615 WP_014779195.1 hypothetical protein -
  THIVI_RS13840 (Thivi_2852) pilM 3135373..3136464 (+) 1092 WP_014779196.1 pilus assembly protein PilM Machinery gene
  THIVI_RS13845 (Thivi_2853) - 3136465..3137112 (+) 648 WP_014779197.1 PilN domain-containing protein -
  THIVI_RS13850 (Thivi_2854) - 3137103..3137720 (+) 618 WP_014779198.1 type 4a pilus biogenesis protein PilO -
  THIVI_RS13855 (Thivi_2855) - 3137717..3138241 (+) 525 WP_014779199.1 pilus assembly protein PilP -
  THIVI_RS13860 (Thivi_2856) - 3138261..3140645 (+) 2385 WP_014779200.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 39476.13 Da        Isoelectric Point: 4.7895

>NTDB_id=51652 THIVI_RS13840 WP_014779196.1 3135373..3136464(+) (pilM) [Thiocystis violascens DSM 198]
MFGLSRKNNPLLGIDISSTAIKLIELSRNAVSAPTLFRVEHYAIEPLPLNAVLEKKIADSEAVGKSIRSALVRAGTKTKR
AAIAVAGSAVITKVISMSASFSDAEMESQIQLEADQYIPYPLEEVNLDFEVIGPSLNSPDMVDVLLAASRRENVDDRVAA
LEIAGLTAIVVDVEAYAMENACSLFPRSGAEESHDRTVALVDVGAATTTLHVLHRGQIIYTREQNFGGMQLKDEVQRRYG
LSREEASQKILTGDVAETYEAEVLDPFKEALAQQIGRALQFFYSGTTFNHVDQILLSGGPASIRKIDVLVENRLKLPTLV
ANPFTQMSLAPSIDSQELMREAPGMMVAVGLALRGFDDPRGFK

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=51652 THIVI_RS13840 WP_014779196.1 3135373..3136464(+) (pilM) [Thiocystis violascens DSM 198]
ATGTTTGGCCTCAGCCGCAAAAATAATCCCCTCCTGGGGATCGACATCAGTTCGACCGCGATCAAATTGATCGAGCTTTC
CAGAAATGCGGTTTCCGCCCCGACGTTGTTTCGGGTCGAGCACTACGCCATCGAGCCGCTCCCCCTGAATGCCGTGCTCG
AAAAAAAAATTGCGGATTCCGAAGCGGTCGGTAAGAGCATCCGTAGCGCCTTGGTCAGGGCTGGAACCAAGACTAAGCGC
GCGGCCATTGCGGTGGCGGGCTCGGCGGTCATTACAAAGGTGATCTCGATGTCGGCCTCGTTCAGCGATGCCGAAATGGA
AAGCCAGATTCAACTGGAGGCTGATCAATACATTCCCTATCCCCTGGAAGAAGTCAATCTCGACTTCGAGGTGATTGGGC
CATCCCTGAACAGCCCCGACATGGTGGACGTTCTGCTGGCCGCCTCCCGCCGGGAAAATGTGGACGATCGCGTTGCCGCG
CTTGAAATCGCCGGTTTGACGGCGATCGTGGTGGATGTCGAAGCCTATGCCATGGAGAATGCCTGTTCGCTGTTTCCGAG
GTCTGGCGCCGAGGAATCTCATGATCGCACGGTGGCGCTCGTGGATGTCGGCGCGGCGACCACGACGCTGCATGTTCTGC
ACCGGGGCCAAATTATCTATACCCGCGAGCAGAACTTCGGTGGCATGCAGTTGAAAGACGAAGTGCAGCGTCGCTATGGT
CTCTCGCGCGAGGAGGCCAGCCAGAAAATCCTGACCGGCGACGTCGCCGAGACCTACGAGGCGGAGGTGCTCGATCCGTT
CAAAGAGGCGCTGGCGCAACAGATCGGACGCGCGCTCCAATTTTTTTACTCTGGAACGACCTTCAACCATGTCGACCAGA
TCCTGCTCTCCGGCGGTCCGGCCAGCATCCGTAAAATCGATGTCCTGGTCGAAAATCGGCTCAAACTGCCGACCCTTGTC
GCGAATCCCTTTACCCAGATGTCTCTTGCGCCCAGCATCGATTCCCAGGAACTGATGCGCGAGGCGCCCGGCATGATGGT
CGCCGTGGGTCTCGCCCTGCGAGGATTCGACGATCCGCGAGGATTCAAATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3YCP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.895

99.725

0.488
  comM Acinetobacter nosocomialis M2

48.895

99.725

0.488
  comM Acinetobacter baylyi ADP1

46.328

97.521

0.452
  pilM Legionella pneumophila strain ERS1305867

42.165

96.694

0.408

Multiple sequence alignment