Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   I6H44_RS02650 Genome accession   NZ_CP065983
Coordinates   562715..564139 (+) Length   474 a.a.
NCBI ID   WP_005715894.1    Uniprot ID   E6KVL9
Organism   Aggregatibacter segnis strain FDAARGOS_987     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 557715..569139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H44_RS02625 (I6H44_02625) - 557760..560327 (-) 2568 WP_005715905.1 penicillin-binding protein 1A -
  I6H44_RS02630 (I6H44_02630) - 560438..561259 (+) 822 WP_005715903.1 hypothetical protein -
  I6H44_RS02635 (I6H44_02635) - 561256..561774 (+) 519 WP_005715900.1 PilN domain-containing protein -
  I6H44_RS02640 (I6H44_02640) - 561771..562298 (+) 528 WP_005715897.1 hypothetical protein -
  I6H44_RS02645 (I6H44_02645) - 562309..562695 (+) 387 WP_005715896.1 pilus assembly protein PilP -
  I6H44_RS02650 (I6H44_02650) comE 562715..564139 (+) 1425 WP_005715894.1 type IV pilus secretin PilQ Machinery gene
  I6H44_RS02655 (I6H44_02655) aroK 564354..564881 (+) 528 WP_005715891.1 shikimate kinase AroK -
  I6H44_RS02660 (I6H44_02660) aroB 564915..566003 (+) 1089 WP_005715889.1 3-dehydroquinate synthase -
  I6H44_RS02665 (I6H44_02665) - 566008..566874 (+) 867 WP_005715888.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  I6H44_RS02670 (I6H44_02670) - 566973..567551 (-) 579 WP_005715886.1 NAD(P)H-dependent oxidoreductase -
  I6H44_RS02675 (I6H44_02675) - 567859..568230 (+) 372 WP_005715885.1 hypothetical protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52034.84 Da        Isoelectric Point: 7.2242

>NTDB_id=515926 I6H44_RS02650 WP_005715894.1 562715..564139(+) (comE) [Aggregatibacter segnis strain FDAARGOS_987]
MQSFFLKILKCGLFFGCFFTFAVHAEQHQTFSIQLKQAPLVPTLQQLALAQNVNLLIDDELQGTLTLKLDNVDLDQLFRS
VAKIKQLDLWQEEGIYYLSKRDTVAKFTGNMTEPQAIAALPSEEETSLATATIKLHFAKASEVMKSLTGGSGSMLSPNGS
ITFDDRSNLLLIQDEPKSIANLKKLIKELDKPIEQIAIEARIVTINAESLKDLGVRWGIFNPTENAHKIAGSLAANGFDN
IANQLNVNLPTATTPAGSIALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTELPYIMVNEKSGTQ
SVEFREAVLGLEVTPHISLDKQILLDLIVSQNAPGSRVAYGLGEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSLD
KVPLLGDIPGIKHLFSKESDRHQKRELVIFVTPHILRQGETLENLKNKQSTAKKNNSLGKSKAAQNTPKKTITQ

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=515926 I6H44_RS02650 WP_005715894.1 562715..564139(+) (comE) [Aggregatibacter segnis strain FDAARGOS_987]
ATGCAATCGTTTTTTCTCAAGATCTTAAAGTGCGGTCTGTTTTTCGGATGTTTTTTCACGTTCGCCGTGCATGCCGAACA
ACATCAAACCTTTTCCATTCAACTCAAACAGGCGCCTTTGGTGCCGACCTTACAACAACTTGCGCTGGCGCAAAATGTTA
ACTTGCTAATTGACGACGAATTGCAAGGCACACTGACATTAAAGTTAGATAACGTAGATTTAGATCAACTCTTTCGTTCC
GTTGCCAAAATCAAACAATTAGATTTATGGCAGGAAGAGGGGATTTATTATTTAAGTAAACGCGATACCGTCGCCAAATT
TACCGGCAATATGACAGAACCTCAAGCCATTGCGGCACTGCCTTCGGAGGAAGAAACCTCGCTCGCGACCGCCACCATTA
AACTGCACTTTGCCAAAGCCTCTGAAGTGATGAAATCACTCACCGGTGGCAGTGGGTCGATGTTATCACCGAATGGCAGT
ATTACCTTTGATGACCGAAGCAACCTCTTGCTGATTCAAGACGAACCGAAATCCATCGCCAATTTGAAAAAATTGATTAA
GGAATTAGATAAGCCCATTGAACAAATCGCCATCGAAGCACGTATTGTCACCATCAACGCGGAAAGTTTGAAAGATTTGG
GCGTGCGTTGGGGGATTTTTAACCCGACAGAAAATGCCCACAAAATTGCTGGCAGTCTCGCCGCTAACGGCTTTGACAAT
ATTGCGAATCAATTAAACGTAAACCTGCCCACCGCCACAACACCGGCGGGGTCTATCGCGTTACAGGTAGCCAAAATCAA
CGGACGACTTTTAGACTTAGAATTGACCGCACTTGAACGGGAAAACAATGTGGAAATTATCGCTAGCCCACGTTTGCTTA
CCACCAACAAGAAAAGCGCCAGCATTAAGCAAGGGACGGAATTGCCTTATATCATGGTGAATGAAAAAAGTGGTACGCAA
AGCGTAGAGTTTCGCGAAGCCGTATTAGGGTTGGAAGTCACACCGCACATTTCCTTGGATAAGCAAATTCTGTTGGATTT
AATTGTCAGTCAAAACGCACCGGGCAGCCGCGTGGCATACGGTTTAGGCGAGGTGGTTTCGATTGATAAACAAGAAATTA
ATACGCAAGTTTTCGCCAAGGATGGGGAAACCATCGTGCTTGGTGGCGTCTTTCACGACACCATTACCAAAAGCTTAGAC
AAAGTGCCATTACTGGGAGACATTCCCGGCATTAAGCATTTGTTTAGCAAAGAAAGTGATCGCCACCAAAAGCGGGAATT
GGTGATCTTCGTCACGCCGCATATTTTGCGCCAAGGTGAGACATTGGAAAACTTGAAGAACAAACAGTCAACAGCGAAAA
AGAACAATAGTCTAGGAAAATCTAAGGCGGCACAAAACACACCTAAAAAGACGATAACGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(277-437)

Bacterial type II/III secretion system short domain (Secretin_N)

(130-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6KVL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

69.584

96.414

0.671
  comE Haemophilus influenzae 86-028NP

68.709

96.414

0.662
  comE Glaesserella parasuis strain SC1401

53.647

89.662

0.481
  pilQ Vibrio campbellii strain DS40M4

42.459

90.928

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.093

90.717

0.382
  pilQ Vibrio cholerae strain A1552

42.093

90.717

0.382