Detailed information NTG0514888

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KYX50_RS14120
• Genome accession: NZ_CP079743
• Coordinates: 2949131..2950249 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 04117201
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2944131..2955249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KYX50_RS14110 (KYX50_14040)	-	2947247..2947939 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
KYX50_RS14115 (KYX50_14045)	pilT	2948066..2949103 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KYX50_RS14120 (KYX50_14050)	pilU	2949131..2950249 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KYX50_RS14125 (KYX50_14055)	fur	2950319..2950756 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KYX50_RS14130 (KYX50_14060)	bamE	2950868..2951266 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KYX50_RS14135 (KYX50_14065)	-	2951322..2951645 (-)	324	WP_000442587.1	RnfH family protein	-
KYX50_RS14140 (KYX50_14070)	-	2951645..2952712 (-)	1068	WP_002019572.1	hypothetical protein	-
KYX50_RS14145 (KYX50_14075)	-	2952865..2953320 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KYX50_RS14150 (KYX50_14080)	-	2953573..2954421 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=514888 KYX50_RS14120 WP_000347039.1 2949131..2950249(+) (pilU) [Acinetobacter baumannii strain 04117201]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=514888 KYX50_RS14120 WP_000347039.1 2949131..2950249(+) (pilU) [Acinetobacter baumannii strain 04117201]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395