Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   STMM8_RS06535 Genome accession   NZ_CP065787
Coordinates   1260326..1262293 (-) Length   655 a.a.
NCBI ID   WP_096811568.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain MM8     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1236695..1306689 1260326..1262293 within 0
IScluster/Tn 1259051..1266389 1260326..1262293 within 0


Gene organization within MGE regions


Location: 1236695..1306689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STMM8_RS06430 (STMM8_06430) liaF 1236703..1237401 (-) 699 WP_011226289.1 cell wall-active antibiotics response protein LiaF -
  STMM8_RS06435 - 1237656..1237823 (-) 168 WP_232089277.1 ion channel -
  STMM8_RS06440 (STMM8_06440) stkP/pknB 1238460..1240331 (-) 1872 WP_260214704.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  STMM8_RS06445 (STMM8_06445) - 1240331..1241068 (-) 738 WP_002946881.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  STMM8_RS06450 (STMM8_06450) rsmB 1241112..1242434 (-) 1323 WP_014608533.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  STMM8_RS06455 (STMM8_06455) fmt 1242424..1243359 (-) 936 WP_002946883.1 methionyl-tRNA formyltransferase -
  STMM8_RS06460 (STMM8_06460) - 1243377..1245773 (-) 2397 WP_176243306.1 primosomal protein N' -
  STMM8_RS06465 (STMM8_06465) rpoZ 1245915..1246229 (-) 315 WP_002951415.1 DNA-directed RNA polymerase subunit omega -
  STMM8_RS06470 (STMM8_06470) gmk 1246251..1246880 (-) 630 WP_022096878.1 guanylate kinase -
  STMM8_RS06475 (STMM8_06475) ftsY 1247113..1248504 (-) 1392 WP_011681410.1 signal recognition particle-docking protein FtsY -
  STMM8_RS06480 (STMM8_06480) - 1248515..1249333 (-) 819 WP_011681411.1 Cof-type HAD-IIB family hydrolase -
  STMM8_RS06485 (STMM8_06485) - 1249326..1250120 (-) 795 WP_022096877.1 HAD-IIB family hydrolase -
  STMM8_RS06490 (STMM8_06490) - 1250304..1250681 (+) 378 WP_011226300.1 GntR family transcriptional regulator -
  STMM8_RS06495 (STMM8_06495) - 1250686..1251384 (+) 699 WP_022096876.1 ABC transporter ATP-binding protein -
  STMM8_RS06500 (STMM8_06500) - 1251396..1252181 (+) 786 WP_002951425.1 hypothetical protein -
  STMM8_RS06505 (STMM8_06505) amiF 1252231..1253160 (-) 930 WP_002951426.1 ATP-binding cassette domain-containing protein Regulator
  STMM8_RS06510 (STMM8_06510) amiE 1253153..1254238 (-) 1086 WP_011226304.1 ABC transporter ATP-binding protein Regulator
  STMM8_RS06515 (STMM8_06515) amiD 1254248..1255174 (-) 927 WP_002946409.1 oligopeptide ABC transporter permease OppC Regulator
  STMM8_RS06520 (STMM8_06520) amiC 1255174..1256667 (-) 1494 WP_002946410.1 ABC transporter permease Regulator
  STMM8_RS06525 (STMM8_06525) amiA 1256729..1258696 (-) 1968 WP_224112099.1 peptide ABC transporter substrate-binding protein Regulator
  STMM8_RS06530 (STMM8_06530) - 1259051..1260281 (+) 1231 Protein_1231 ISL3 family transposase -
  STMM8_RS06535 (STMM8_06535) amiA 1260326..1262293 (-) 1968 WP_096811568.1 peptide ABC transporter substrate-binding protein Regulator
  STMM8_RS06540 (STMM8_06540) - 1262497..1262687 (-) 191 Protein_1233 IS3 family transposase -
  STMM8_RS06545 - 1262681..1263091 (-) 411 Protein_1234 ATP-binding cassette domain-containing protein -
  STMM8_RS06550 (STMM8_06555) - 1263123..1263437 (-) 315 Protein_1235 TatD family hydrolase -
  STMM8_RS06555 (STMM8_06560) - 1263786..1265036 (+) 1251 WP_405384965.1 OFA family MFS transporter -
  STMM8_RS06560 (STMM8_06565) - 1265037..1266389 (-) 1353 Protein_1237 IS3 family transposase -
  STMM8_RS06565 (STMM8_06570) pta 1266475..1267458 (-) 984 WP_022097032.1 phosphate acetyltransferase -
  STMM8_RS06570 (STMM8_06575) - 1267472..1268368 (-) 897 WP_011226316.1 RluA family pseudouridine synthase -
  STMM8_RS06575 (STMM8_06580) - 1268365..1269201 (-) 837 WP_022097031.1 NAD kinase -
  STMM8_RS06580 (STMM8_06585) - 1269173..1269847 (-) 675 WP_002951443.1 GTP pyrophosphokinase family protein -
  STMM8_RS06585 (STMM8_06590) - 1269943..1270518 (+) 576 WP_002951444.1 CYTH domain-containing protein -
  STMM8_RS06590 (STMM8_06595) - 1270881..1271852 (+) 972 WP_002951446.1 ribose-phosphate diphosphokinase -
  STMM8_RS06595 (STMM8_06600) - 1271856..1272968 (+) 1113 WP_002948029.1 cysteine desulfurase family protein -
  STMM8_RS06600 (STMM8_06605) - 1272970..1273317 (+) 348 WP_002948028.1 DUF1831 domain-containing protein -
  STMM8_RS06605 (STMM8_06610) - 1273461..1274120 (+) 660 WP_002948027.1 redox-sensing transcriptional repressor Rex -
  STMM8_RS06610 (STMM8_06615) - 1274113..1274808 (+) 696 WP_002948026.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  STMM8_RS06615 (STMM8_06620) radC 1274861..1275547 (-) 687 WP_002948025.1 DNA repair protein RadC -
  STMM8_RS06620 (STMM8_06625) - 1275596..1277376 (-) 1781 Protein_1249 rhamnan synthesis F family protein -
  STMM8_RS06625 (STMM8_06630) - 1277373..1278418 (-) 1046 Protein_1250 glycosyltransferase family 2 protein -
  STMM8_RS06630 (STMM8_06635) - 1278393..1279629 (-) 1237 Protein_1251 ABC transporter ATP-binding protein -
  STMM8_RS06635 - 1279629..1280431 (-) 803 Protein_1252 ABC transporter permease -
  STMM8_RS06640 (STMM8_06645) - 1280415..1281361 (-) 947 Protein_1253 glycosyltransferase family 2 protein -
  STMM8_RS06645 (STMM8_06650) cps2T 1281358..1282502 (-) 1145 Protein_1254 beta 1-4 rhamnosyltransferase Cps2T -
  STMM8_RS06650 (STMM8_06655) - 1282611..1283621 (-) 1011 WP_022097029.1 glycosyltransferase family A protein -
  STMM8_RS06655 (STMM8_06660) - 1283621..1284895 (-) 1275 WP_022097028.1 lipopolysaccharide biosynthesis protein -
  STMM8_RS06660 (STMM8_06665) - 1284888..1285220 (-) 333 WP_011226332.1 DUF2304 domain-containing protein -
  STMM8_RS06665 (STMM8_06670) - 1285226..1285921 (-) 696 WP_173940715.1 glycosyltransferase family 2 protein -
  STMM8_RS06670 (STMM8_06675) rfbD 1285998..1286849 (-) 852 WP_022097027.1 dTDP-4-dehydrorhamnose reductase -
  STMM8_RS06675 (STMM8_06680) - 1286926..1288260 (-) 1335 WP_002946422.1 DUF6056 family protein -
  STMM8_RS06680 (STMM8_06685) - 1288373..1289290 (-) 918 WP_002946425.1 glycosyltransferase family 2 protein -
  STMM8_RS06685 (STMM8_06690) - 1289353..1290768 (-) 1416 WP_260214684.1 DUF2142 domain-containing protein -
  STMM8_RS06690 (STMM8_06695) - 1291102..1291437 (-) 336 WP_002891474.1 metal-sulfur cluster assembly factor -
  STMM8_RS06695 (STMM8_06700) rpoD 1291491..1292600 (-) 1110 WP_011226338.1 RNA polymerase sigma factor RpoD -
  STMM8_RS06700 (STMM8_06705) dnaG 1292604..1294415 (-) 1812 WP_011681448.1 DNA primase -
  STMM8_RS06705 (STMM8_06710) mscL 1294556..1294639 (+) 84 Protein_1266 large conductance mechanosensitive channel protein MscL -
  STMM8_RS06710 (STMM8_06715) rpsU 1294799..1294975 (-) 177 WP_082309068.1 30S ribosomal protein S21 -
  STMM8_RS06715 (STMM8_06720) - 1295170..1295964 (-) 795 WP_022097025.1 ABC transporter substrate-binding protein -
  STMM8_RS06720 (STMM8_06725) - 1296026..1297135 (-) 1110 WP_022097024.1 aminotransferase -
  STMM8_RS06725 (STMM8_06730) - 1297481..1298278 (-) 798 WP_022097023.1 transporter substrate-binding domain-containing protein -
  STMM8_RS06730 (STMM8_06735) - 1298275..1299105 (-) 831 WP_022097022.1 transporter substrate-binding domain-containing protein -
  STMM8_RS06735 (STMM8_06740) - 1299319..1299783 (-) 465 WP_011681454.1 8-oxo-dGTP diphosphatase -
  STMM8_RS06740 (STMM8_06745) uvrB 1299828..1301819 (-) 1992 WP_002953358.1 excinuclease ABC subunit UvrB -
  STMM8_RS06745 (STMM8_06750) - 1301966..1302433 (-) 468 WP_022097021.1 hypothetical protein -
  STMM8_RS06750 (STMM8_06755) - 1302506..1303442 (-) 937 Protein_1275 CPBP family intramembrane glutamic endopeptidase -
  STMM8_RS06755 (STMM8_06760) - 1303641..1305851 (+) 2211 WP_173940613.1 ABC transporter substrate-binding protein/permease -
  STMM8_RS06760 (STMM8_06765) - 1305851..1306591 (+) 741 WP_002945131.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 71600.64 Da        Isoelectric Point: 5.1811

>NTDB_id=514274 STMM8_RS06535 WP_096811568.1 1260326..1262293(-) (amiA) [Streptococcus thermophilus strain MM8]
MKKSKWLALAGVALLGVGVLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQDGLYNAKKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMQN
ATSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKAFNGYDDPNNAAAAQVGLKDYDALLDSAASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKEKK
ASNEKAQKDLEKHVK

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=514274 STMM8_RS06535 WP_096811568.1 1260326..1262293(-) (amiA) [Streptococcus thermophilus strain MM8]
ATGAAAAAAAGCAAATGGTTGGCCCTCGCGGGTGTAGCACTTCTAGGTGTTGGTGTGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTGACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCCGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATCGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGTGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGACGGTCTCTACAATGCTAAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGATATGCAAAAT
GCGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGATTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGGCTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAGGATTATGATGCCTTGCTTGATTCAGCAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAAGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

90.091

100

0.902
  amiA3 Streptococcus thermophilus LMD-9

88.128

100

0.884
  amiA3 Streptococcus thermophilus LMG 18311

87.823

100

0.881