Detailed information NTG0513451

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6G34_RS03535
• Genome accession: NZ_CP065720
• Coordinates: 789470..790615 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain FDAARGOS_877
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 784470..795615

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G34_RS03510 (I6G34_03510)	-	784549..785565 (+)	1017	WP_017245430.1	aspartate carbamoyltransferase catalytic subunit	-
I6G34_RS03515 (I6G34_03515)	-	785565..786839 (+)	1275	WP_102838899.1	dihydroorotase	-
I6G34_RS03520 (I6G34_03520)	-	786897..787292 (-)	396	WP_017245432.1	TM2 domain-containing protein	-
I6G34_RS03525 (I6G34_03525)	-	787425..788639 (+)	1215	WP_102838900.1	putative DNA modification/repair radical SAM protein	-
I6G34_RS03530 (I6G34_03530)	-	788639..789463 (+)	825	WP_102838901.1	TIGR03915 family putative DNA repair protein	-
I6G34_RS03535 (I6G34_03535)	pilU	789470..790615 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6G34_RS03540 (I6G34_03540)	pilT	790676..791710 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
I6G34_RS03545 (I6G34_03545)	-	791777..792466 (+)	690	WP_102838902.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6G34_RS03550 (I6G34_03550)	proC	792477..793301 (+)	825	WP_102838903.1	pyrroline-5-carboxylate reductase	-
I6G34_RS03555 (I6G34_03555)	-	793311..793901 (+)	591	WP_102838904.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=513451 I6G34_RS03535 WP_026006585.1 789470..790615(-) (pilU) [Stutzerimonas frequens strain FDAARGOS_877]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=513451 I6G34_RS03535 WP_026006585.1 789470..790615(-) (pilU) [Stutzerimonas frequens strain FDAARGOS_877]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCGGGCGTGCCGCCCTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAAAATCACGAATGCAACTTCGCCATCAGTGCCCGTGGGATCGGTCGC
TTCCGTGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATTCCGACAATCGA
AGAGCTGAAGCTGCCTGACGTTCTCAAGAAGCTGTCGATGACCAAGCGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACAATGGACTATG
CCGTAGCGTTCGCCGAAACCGGACACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAAGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTAATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCACCGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388