Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KUU74_RS28040 Genome accession   NZ_CP078009
Coordinates   6014984..6015607 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain FAHZU31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6009984..6020607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUU74_RS28020 (KUU74_28015) - 6011985..6012071 (-) 87 Protein_5565 pilus assembly protein PilP -
  KUU74_RS28025 (KUU74_28020) - 6012422..6013147 (+) 726 WP_203023644.1 Bro-N domain-containing protein -
  KUU74_RS28030 (KUU74_28025) - 6013144..6014508 (+) 1365 WP_203023646.1 ISL3 family transposase -
  KUU74_RS28035 (KUU74_28030) pilP 6014532..6014987 (-) 456 Protein_5568 type 4a pilus biogenesis lipoprotein PilP -
  KUU74_RS28040 (KUU74_28035) pilO 6014984..6015607 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  KUU74_RS28045 (KUU74_28040) pilN 6015604..6016200 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  KUU74_RS28050 (KUU74_28045) pilM 6016200..6017264 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  KUU74_RS28055 (KUU74_28050) - 6017449..6019920 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=513415 KUU74_RS28040 WP_003103268.1 6014984..6015607(-) (pilO) [Pseudomonas aeruginosa strain FAHZU31]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=513415 KUU74_RS28040 WP_003103268.1 6014984..6015607(-) (pilO) [Pseudomonas aeruginosa strain FAHZU31]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401