Detailed information NTG0513009

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: I6G50_RS02685
• Genome accession: NZ_CP065691
• Coordinates: 504763..506019 (-)
• Length: 418 a.a.
• NCBI ID: WP_197909403.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain FDAARGOS_893
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 499763..511019

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G50_RS02650 (I6G50_02650)	rpmA	499913..500197 (+)	285	WP_003136340.1	50S ribosomal protein L27	-
I6G50_RS02655 (I6G50_02655)	-	500510..501616 (+)	1107	WP_197909075.1	alanine dehydrogenase	-
I6G50_RS02660 (I6G50_02660)	-	501743..502702 (+)	960	WP_081167613.1	PhoH family protein	-
I6G50_RS02665 (I6G50_02665)	-	502704..503177 (+)	474	WP_003136348.1	NUDIX hydrolase	-
I6G50_RS02670 (I6G50_02670)	ybeY	503187..503675 (+)	489	WP_003136350.1	rRNA maturation RNase YbeY	-
I6G50_RS02675 (I6G50_02675)	-	503659..504120 (+)	462	WP_269668695.1	diacylglycerol kinase family protein	-
I6G50_RS02680 (I6G50_02680)	comFC/cflB	504122..504766 (-)	645	WP_197909076.1	ComF family protein	Machinery gene
I6G50_RS02685 (I6G50_02685)	comFA	504763..506019 (-)	1257	WP_197909403.1	DEAD/DEAH box helicase	Machinery gene
I6G50_RS02690 (I6G50_02690)	-	506248..506871 (+)	624	WP_197909077.1	YigZ family protein	-
I6G50_RS02695 (I6G50_02695)	-	506884..507906 (+)	1023	WP_003136363.1	DUF475 domain-containing protein	-
I6G50_RS02700 (I6G50_02700)	-	508233..509030 (+)	798	WP_003136365.1	formate/nitrite transporter family protein	-
I6G50_RS02705 (I6G50_02705)	glyQ	509020..509988 (+)	969	WP_003136366.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47966.08 Da
• Isoelectric Point: 10.3250

>NTDB_id=513009 I6G50_RS02685 WP_197909403.1 504763..506019(-) (comFA) [Lactococcus garvieae strain FDAARGOS_893]
MDELYGRLLLKKDLKTVPSQAIVFESMNKITKSKMRCNRCGQVSEEKLIRLPIGYHYCPHCIQMGRVRSDEKLYHLPQQD
FEGASYLRWQGNLTTLQKDISERLIQFSHTHKNILVQAVTGAGKTEMIYPIINEALKKGKAVGLTSPRIDVCLELHRRLS
RDFSCPIALLHGQGEAYTRTPLVIATTHQLLRFHAAFELLILDEVDAFPFTDNKMLTFALNKARTDTSRLVYLTATPTNE
LEKQVKCGQIKKLQLARRFHGFPLVLPQFIWQSKFYFKLKEQRATAFPLLIFVPKIKQGEELLQILKKAFPKEAIAFVAS
TSIDRLDSVEKFRKGEISILISTTILERGVTFPKVDVFVFHSHHHNFTSSSLIQIAGRVGRSTDRPEGKVYFFHQGKTKR
MVEAYRNIKKMNQIGGFI

Nucleotide

• Length: 1257 bp

>NTDB_id=513009 I6G50_RS02685 WP_197909403.1 504763..506019(-) (comFA) [Lactococcus garvieae strain FDAARGOS_893]
ATGGATGAATTATATGGGAGACTCCTCCTAAAAAAAGATTTAAAAACTGTTCCTAGTCAAGCTATCGTTTTTGAGAGTAT
GAATAAAATAACCAAAAGCAAAATGAGATGCAATCGCTGTGGTCAAGTTAGCGAAGAAAAGTTAATTAGGCTCCCTATAG
GGTATCATTATTGCCCGCATTGTATCCAAATGGGTCGTGTTCGCTCAGACGAGAAGCTTTACCACCTCCCTCAACAAGAT
TTTGAAGGTGCTTCTTACCTCAGGTGGCAAGGTAACTTGACTACACTACAAAAAGATATATCTGAACGTTTGATCCAGTT
CAGTCACACTCATAAAAATATTCTCGTCCAAGCTGTGACAGGTGCTGGAAAGACCGAAATGATATACCCAATAATCAATG
AAGCTTTAAAAAAAGGAAAGGCTGTTGGACTTACGAGTCCTCGAATCGATGTTTGTCTAGAACTTCACCGTCGCTTAAGT
CGAGACTTTTCTTGTCCTATCGCCCTTTTGCATGGTCAAGGTGAGGCCTATACACGCACACCACTTGTTATTGCGACTAC
ACATCAGCTTTTGCGTTTTCATGCTGCCTTTGAATTGCTTATTTTGGACGAAGTTGATGCTTTTCCTTTTACGGATAACA
AAATGCTTACTTTTGCTCTTAACAAAGCGCGAACAGATACTTCTCGATTAGTTTACCTCACAGCGACACCTACAAATGAG
TTAGAAAAACAAGTTAAGTGTGGACAGATAAAAAAATTGCAACTGGCACGCCGTTTTCACGGTTTCCCCCTTGTACTGCC
TCAATTTATTTGGCAAAGTAAGTTTTACTTTAAGCTTAAAGAGCAGAGAGCTACAGCTTTTCCCCTTCTCATATTTGTCC
CCAAAATAAAACAAGGGGAGGAACTTCTTCAAATTTTAAAAAAAGCTTTCCCTAAGGAAGCTATTGCTTTTGTGGCTTCT
ACAAGCATCGATCGACTTGATAGTGTAGAAAAATTTCGAAAAGGAGAAATTTCTATTCTCATTTCAACGACAATACTCGA
ACGTGGTGTAACTTTTCCGAAAGTCGATGTTTTTGTTTTTCATAGTCATCACCATAATTTCACAAGTTCAAGTTTAATTC
AAATTGCTGGACGTGTAGGTCGTAGTACAGATAGGCCAGAAGGTAAAGTCTATTTTTTTCATCAGGGAAAAACAAAAAGG
ATGGTAGAGGCCTACAGAAATATAAAGAAAATGAATCAAATTGGAGGATTTATATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (92-238)

• Helicase conserved C-terminal domain (Helicase_C) (288-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.908	93.78	0.543
comFA/cflA	Streptococcus pneumoniae TIGR4	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae Rx1	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae D39	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae R6	52.113	100	0.531
comFA/cflA	Streptococcus mitis SK321	50.469	100	0.514
comFA/cflA	Streptococcus mitis NCTC 12261	49.765	100	0.507
comFA	Bacillus subtilis subsp. subtilis str. 168	38.005	100	0.383
comFA	Latilactobacillus sakei subsp. sakei 23K	37.838	97.368	0.368