Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D7S_RS08005 Genome accession   NC_017846
Coordinates   1612740..1613192 (-) Length   150 a.a.
NCBI ID   WP_005550263.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans D7S-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1607740..1618192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7S_RS07980 (D7S_03395) - 1607894..1608781 (-) 888 WP_014702484.1 DUF4123 domain-containing protein -
  D7S_RS07985 - 1609056..1609226 (-) 171 WP_014702486.1 hypothetical protein -
  D7S_RS07990 (D7S_01720) - 1609402..1610091 (-) 690 WP_005594882.1 prepilin peptidase -
  D7S_RS07995 (D7S_01721) pilC 1610088..1611311 (-) 1224 WP_005540455.1 type II secretion system F family protein Machinery gene
  D7S_RS08000 (D7S_01722) pilB 1611304..1612713 (-) 1410 WP_005540457.1 GspE/PulE family protein Machinery gene
  D7S_RS08005 (D7S_01723) pilA 1612740..1613192 (-) 453 WP_005550263.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D7S_RS08010 (D7S_01724) ampD 1613314..1613868 (+) 555 WP_005540460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D7S_RS08015 (D7S_01726) nanQ 1614334..1614807 (-) 474 WP_005594879.1 N-acetylneuraminate anomerase -
  D7S_RS08020 (D7S_01727) pnp 1615101..1617230 (+) 2130 WP_032997160.1 polyribonucleotide nucleotidyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15503.95 Da        Isoelectric Point: 9.4349

>NTDB_id=50973 D7S_RS08005 WP_005550263.1 1612740..1613192(-) (pilA) [Aggregatibacter actinomycetemcomitans D7S-1]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAATGVSWNAACGNNAGIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=50973 D7S_RS08005 WP_005550263.1 1612740..1613192(-) (pilA) [Aggregatibacter actinomycetemcomitans D7S-1]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCGGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCACCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGGCATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.043

92

0.58
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513

Multiple sequence alignment