Detailed information NTG0509618

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I5593_RS14640
• Genome accession: NZ_CP065432
• Coordinates: 3049402..3050520 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain ATCC 17961
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3044402..3055520

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5593_RS14630 (I5593_14630)	-	3047518..3048210 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
I5593_RS14635 (I5593_14635)	pilT	3048337..3049374 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
I5593_RS14640 (I5593_14640)	pilU	3049402..3050520 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I5593_RS14645 (I5593_14645)	fur	3050590..3051027 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
I5593_RS14650 (I5593_14650)	-	3051139..3051537 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
I5593_RS14655 (I5593_14655)	-	3051592..3051915 (-)	324	WP_000442584.1	RnfH family protein	-
I5593_RS14660 (I5593_14660)	-	3051915..3052982 (-)	1068	WP_029642282.1	membrane protein	-
I5593_RS14665 (I5593_14665)	-	3053135..3053590 (-)	456	WP_000782976.1	bacteriohemerythrin	-
I5593_RS14670 (I5593_14670)	-	3053843..3054691 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=509618 I5593_RS14640 WP_000347036.1 3049402..3050520(+) (pilU) [Acinetobacter baumannii strain ATCC 17961]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=509618 I5593_RS14640 WP_000347036.1 3049402..3050520(+) (pilU) [Acinetobacter baumannii strain ATCC 17961]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392