Detailed information NTG0050830

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NGFG_RS10620
• Genome accession: NC_022240
• Coordinates: 1953091..1954317 (+)
• Length: 408 a.a.
• NCBI ID: WP_003690199.1
• Uniprot ID: -
• Organism: Neisseria gonorrhoeae MS11
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1948091..1959317

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NGFG_RS10595 (NGFG_01984)	dksA	1949267..1949683 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
NGFG_RS10600 (NGFG_01983)	proC	1949831..1950622 (-)	792	WP_003696395.1	pyrroline-5-carboxylate reductase	-
NGFG_RS10605 (NGFG_01982)	-	1950671..1951078 (-)	408	WP_003690202.1	hypothetical protein	-
NGFG_RS10610 (NGFG_01981)	-	1951078..1951764 (-)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
NGFG_RS10615 (NGFG_01980)	pilT	1951884..1952927 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
NGFG_RS10620 (NGFG_01979)	pilU	1953091..1954317 (+)	1227	WP_003690199.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NGFG_RS10625 (NGFG_01978)	yccS	1954434..1956584 (+)	2151	WP_003694501.1	YccS family putative transporter	-
NGFG_RS10630 (NGFG_02247)	-	1957514..1957798 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45672.29 Da
• Isoelectric Point: 7.3203

>NTDB_id=50830 NGFG_RS10620 WP_003690199.1 1953091..1954317(+) (pilU) [Neisseria gonorrhoeae MS11]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=50830 NGFG_RS10620 WP_003690199.1 1953091..1954317(+) (pilU) [Neisseria gonorrhoeae MS11]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	44.944	87.255	0.392
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387