Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BTD91_RS07605 Genome accession   NZ_CP065150
Coordinates   1742095..1743120 (+) Length   341 a.a.
NCBI ID   WP_077680948.1    Uniprot ID   -
Organism   Vibrio kanaloae strain R17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737095..1748120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTD91_RS07590 (BTD91_07590) - 1737444..1738172 (-) 729 WP_017055839.1 glutathione peroxidase -
  BTD91_RS07595 (BTD91_07595) oxyR 1738392..1739282 (+) 891 WP_017055838.1 DNA-binding transcriptional regulator OxyR -
  BTD91_RS07600 (BTD91_07600) - 1739382..1741943 (-) 2562 WP_175627321.1 penicillin-binding protein 1A -
  BTD91_RS07605 (BTD91_07605) pilM 1742095..1743120 (+) 1026 WP_077680948.1 type IV pilus assembly protein PilM Machinery gene
  BTD91_RS07610 (BTD91_07610) pilN 1743104..1743703 (+) 600 WP_017055835.1 PilN domain-containing protein Machinery gene
  BTD91_RS07615 (BTD91_07615) pilO 1743696..1744307 (+) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  BTD91_RS07620 (BTD91_07620) pilP 1744294..1744812 (+) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  BTD91_RS07625 (BTD91_07625) pilQ 1744872..1746563 (+) 1692 WP_046224652.1 type IV pilus secretin PilQ Machinery gene
  BTD91_RS07630 (BTD91_07630) aroK 1746740..1747258 (+) 519 WP_017061125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37989.56 Da        Isoelectric Point: 7.2208

>NTDB_id=507424 BTD91_RS07605 WP_077680948.1 1742095..1743120(+) (pilM) [Vibrio kanaloae strain R17]
MGSSLITGIDINHHSIKAVVLKPVGELYAIVGYKELPISDDIFTANHTLEYQKTVKKLKELKKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRANALSKAGFKP
IVVDTQAHGLLNIWQLASYLSPEKKNWLLVDVGIDQTSLGIIPQNSAPFYKDIAFGTQELLGTGSPSDMEGVFGTTQDTH
RFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASIPMVVEELERHFQMSCESLNPMSLFENKVAQKRRSSLNCQHFG
IAAGMAMRGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=507424 BTD91_RS07605 WP_077680948.1 1742095..1743120(+) (pilM) [Vibrio kanaloae strain R17]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGTATCAAAGCTGTGGTACTAAAACCCGTTGGGGAGTT
GTATGCCATCGTGGGATACAAAGAGCTGCCTATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAAGAAAGGACTGCCTTTTGGAAGTCGTAACGTCGCTATTTCAGTACCAGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGATAGAGAGAAAGAGTTTTCTATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAAGAGCTGAGTTTAGATTTTGTAAAGCTCGAAGACAAACGGTTTGGTAAAGGATCGACAA
GTAGTTATCAGGTGTACGCGACTCGCAAGGAAGTTGTGGATAGTCGAGCCAATGCATTAAGCAAAGCCGGGTTTAAACCT
ATCGTCGTTGATACCCAAGCACACGGACTTCTCAATATTTGGCAATTGGCTTCGTATTTGTCCCCAGAAAAGAAGAATTG
GCTGCTGGTGGACGTTGGAATCGACCAAACCTCGTTAGGGATTATCCCGCAGAATTCTGCGCCTTTTTATAAAGACATCG
CCTTTGGTACTCAAGAGCTGTTAGGCACAGGCAGTCCAAGTGATATGGAAGGCGTGTTCGGCACCACTCAGGATACACAT
CGGTTTATTGTTAATCTTATTGAAAAGCTCAAGCGTCAACTGCAACTCTATTCGTCGGTAAATGCGCTTCAACCTATCTC
AGGGATTTGGCTAATGGGCGAAGGAGCGAGCATACCAATGGTCGTTGAGGAGTTAGAACGTCATTTTCAGATGAGTTGTG
AGTCACTGAACCCTATGTCTTTGTTTGAGAATAAGGTGGCCCAGAAACGCCGATCGTCACTGAATTGTCAGCATTTTGGT
ATTGCGGCGGGGATGGCAATGCGTGGTCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-329)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.392

99.413

0.531
  pilM Vibrio campbellii strain DS40M4

47.076

100

0.472