Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NT08PM_RS06145 Genome accession   NC_017764
Coordinates   1320212..1320646 (-) Length   144 a.a.
NCBI ID   WP_014668227.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. multocida str. 3480     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1315212..1325646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT08PM_RS06115 (NT08PM_1250) - 1315776..1316045 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  NT08PM_RS06120 (NT08PM_1251) yacG 1316045..1316248 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  NT08PM_RS06125 (NT08PM_1252) coaE 1316259..1316879 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  NT08PM_RS06130 (NT08PM_1253) - 1316894..1317616 (-) 723 WP_016534448.1 prepilin peptidase -
  NT08PM_RS06135 (NT08PM_1254) - 1317616..1318827 (-) 1212 WP_014668225.1 type II secretion system F family protein -
  NT08PM_RS06140 (NT08PM_1255) pilB 1318827..1320212 (-) 1386 WP_014668226.1 GspE/PulE family protein Machinery gene
  NT08PM_RS06145 (NT08PM_1256) pilA 1320212..1320646 (-) 435 WP_014668227.1 prepilin peptidase-dependent pilin Machinery gene
  NT08PM_RS06150 (NT08PM_1257) ampD 1320807..1321373 (+) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NT08PM_RS06155 (NT08PM_1258) rppH 1321959..1322426 (+) 468 Protein_1193 RNA pyrophosphohydrolase -
  NT08PM_RS06160 (NT08PM_1259) - 1322558..1323352 (+) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  NT08PM_RS06165 (NT08PM_1260) lgt 1323362..1324165 (+) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  NT08PM_RS06170 (NT08PM_1261) - 1324171..1325022 (+) 852 WP_014668231.1 thymidylate synthase -
  NT08PM_RS06175 (NT08PM_1262) tadA 1325049..1325537 (+) 489 WP_014668232.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15084.52 Da        Isoelectric Point: 9.1281

>NTDB_id=50710 NT08PM_RS06145 WP_014668227.1 1320212..1320646(-) (pilA) [Pasteurella multocida subsp. multocida str. 3480]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTPSGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=50710 NT08PM_RS06145 WP_014668227.1 1320212..1320646(-) (pilA) [Pasteurella multocida subsp. multocida str. 3480]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACACCGAGTGGTAACGCTGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA/pilA1 Eikenella corrodens VA1

38.129

96.528

0.368

Multiple sequence alignment