Detailed information NTG0506824

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: VB13_RS00335
• Genome accession: NZ_CP077685
• Coordinates: 54372..55370 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain M49 591
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49372..60370

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VB13_RS00315 (VB13_00330)	-	50159..51640 (+)	1482	WP_009880316.1	PIN-like domain-containing protein	-
VB13_RS00320 (VB13_00335)	purB	51813..53105 (+)	1293	WP_009880315.1	adenylosuccinate lyase	-
VB13_RS00325 (VB13_00340)	rgg4	53236..54146 (+)	911	Protein_31	transcriptional regulator Rgg4/ComR	-
VB13_RS08820 (VB13_00350)	comS	54230..54325 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
VB13_RS00335 (VB13_00355)	ruvB	54372..55370 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
VB13_RS00340 (VB13_00360)	-	55508..55945 (+)	438	WP_012560368.1	low molecular weight protein-tyrosine-phosphatase	-
VB13_RS00345 (VB13_00365)	-	55968..56369 (+)	402	WP_012560369.1	MORN repeat-containing protein	-
VB13_RS00350 (VB13_00370)	-	56366..58141 (+)	1776	WP_009880312.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=506824 VB13_RS00335 WP_002986679.1 54372..55370(+) (ruvB) [Streptococcus pyogenes strain M49 591]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=506824 VB13_RS00335 WP_002986679.1 54372..55370(+) (ruvB) [Streptococcus pyogenes strain M49 591]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATCTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAG

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473