Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IYN86_RS07360 Genome accession   NZ_CP064868
Coordinates   1471414..1472316 (+) Length   300 a.a.
NCBI ID   WP_218697438.1    Uniprot ID   A0AAW5LFX6
Organism   Mammaliicoccus sciuri strain 82104     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466414..1477316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IYN86_RS07320 - 1466498..1467052 (+) 555 WP_096791884.1 5-formyltetrahydrofolate cyclo-ligase -
  IYN86_RS07325 - 1467053..1468501 (+) 1449 WP_096791885.1 rhomboid family intramembrane serine protease -
  IYN86_RS07330 - 1468506..1468697 (+) 192 WP_078099923.1 YqgQ family protein -
  IYN86_RS07335 - 1468694..1469665 (+) 972 WP_218697436.1 ROK family glucokinase -
  IYN86_RS07340 - 1469665..1469979 (+) 315 WP_058611614.1 MTH1187 family thiamine-binding protein -
  IYN86_RS07345 - 1470009..1470248 (-) 240 WP_016911885.1 DUF2759 domain-containing protein -
  IYN86_RS07350 - 1470346..1470978 (+) 633 WP_058611613.1 MBL fold metallo-hydrolase -
  IYN86_RS07355 - 1471012..1471260 (-) 249 WP_025904960.1 DUF2626 family protein -
  IYN86_RS07360 comGA 1471414..1472316 (+) 903 WP_218697438.1 competence type IV pilus ATPase ComGA Machinery gene
  IYN86_RS07365 comGB 1472306..1473346 (+) 1041 WP_196968298.1 competence type IV pilus assembly protein ComGB -
  IYN86_RS07370 comGC 1473361..1473678 (+) 318 WP_058611611.1 competence type IV pilus major pilin ComGC Machinery gene
  IYN86_RS07375 comGD 1473659..1474105 (+) 447 WP_227531649.1 competence type IV pilus minor pilin ComGD -
  IYN86_RS07380 comGE 1474089..1474364 (+) 276 WP_248559359.1 competence type IV pilus minor pilin ComGE -
  IYN86_RS07385 - 1474361..1474780 (+) 420 WP_248559360.1 competence type IV pilus minor pilin ComGF -
  IYN86_RS07390 - 1475015..1475503 (+) 489 WP_196968295.1 shikimate kinase -
  IYN86_RS07395 gcvT 1475696..1476784 (+) 1089 WP_196968294.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34387.26 Da        Isoelectric Point: 6.1198

>NTDB_id=505455 IYN86_RS07360 WP_218697438.1 1471414..1472316(+) (comGA) [Mammaliicoccus sciuri strain 82104]
MEKLFKSIINDAINSNASDIHFVPLNDDKVFVQVRINGDNIPYDLDLDFNLYSRLLIYMKFIAHLDVSERNKAQSGQYLF
EHDIHYYLRISTIPISLGIESSSIRITPQHFNHSQVKSEHQSYNVLQNRMYQSEGLFLFTGPTGSGKTTLMYELMYALKR
EKNKHIITIEDPVEQTLNGIVQVSVNEKANITYQQSFKAILRCDPDVIMIGEIRDDITAKQVIQASLSGHLVLSTMHASD
TIGAIHRLVELGVTVEQIKQGISCITNQRLVKKCDEARTLDMSYIFKDDIIKYMENDYET

Nucleotide


Download         Length: 903 bp        

>NTDB_id=505455 IYN86_RS07360 WP_218697438.1 1471414..1472316(+) (comGA) [Mammaliicoccus sciuri strain 82104]
ATGGAGAAATTATTTAAGTCAATTATTAACGATGCTATCAATTCTAATGCGAGTGATATTCACTTTGTACCTTTAAACGA
TGATAAAGTCTTTGTCCAAGTGAGGATAAATGGCGATAATATACCTTATGATTTAGATTTAGATTTTAATTTGTATAGTA
GGCTACTTATTTATATGAAATTTATCGCTCATCTAGATGTAAGTGAACGAAATAAAGCACAAAGTGGTCAATATTTATTT
GAACATGACATACATTACTATTTAAGAATTTCCACGATACCTATCAGTTTAGGGATAGAATCTAGTTCCATTAGAATAAC
CCCTCAACATTTTAATCATTCACAAGTGAAATCAGAGCATCAATCATATAACGTTCTACAAAACAGAATGTATCAATCAG
AAGGGCTATTTTTATTTACGGGTCCAACTGGATCAGGTAAGACTACATTAATGTATGAACTTATGTACGCATTAAAGAGA
GAGAAGAATAAACATATTATTACGATTGAAGACCCTGTAGAACAAACGTTAAATGGCATCGTTCAAGTTTCAGTCAATGA
AAAAGCTAACATAACTTATCAACAATCCTTTAAAGCCATTTTAAGATGTGATCCGGACGTTATTATGATTGGAGAGATAA
GAGATGATATTACTGCTAAGCAAGTTATTCAAGCTAGTTTAAGTGGTCATTTAGTACTTTCAACAATGCATGCGAGTGAT
ACAATCGGGGCAATCCACCGACTAGTTGAGCTAGGTGTAACAGTCGAACAAATTAAACAAGGTATTTCATGTATTACGAA
CCAACGTCTTGTGAAGAAATGTGATGAAGCAAGAACATTAGATATGTCCTATATTTTTAAAGATGACATTATTAAATATA
TGGAGAATGATTATGAAACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

52.5

93.333

0.49
  comGA Staphylococcus aureus N315

52.5

93.333

0.49
  comGA Bacillus subtilis subsp. subtilis str. 168

40.58

92

0.373
  pilB Haemophilus influenzae Rd KW20

35.032

100

0.367