Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IXK98_RS22895 Genome accession   NZ_CP064858
Coordinates   3076578..3077165 (-) Length   195 a.a.
NCBI ID   WP_171802178.1    Uniprot ID   A0AAE7DXD5
Organism   Vibrio europaeus strain 07/118 T2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3071578..3082165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXK98_RS22875 (IXK98_22875) aroB 3072565..3073653 (-) 1089 WP_171383619.1 3-dehydroquinate synthase -
  IXK98_RS22880 (IXK98_22880) aroK 3073682..3074200 (-) 519 WP_069667329.1 shikimate kinase AroK -
  IXK98_RS22885 (IXK98_22885) pilQ 3074377..3076053 (-) 1677 WP_171802406.1 type IV pilus secretin PilQ Machinery gene
  IXK98_RS22890 (IXK98_22890) pilP 3076085..3076588 (-) 504 WP_171802177.1 pilus assembly protein PilP Machinery gene
  IXK98_RS22895 (IXK98_22895) pilO 3076578..3077165 (-) 588 WP_171802178.1 type 4a pilus biogenesis protein PilO Machinery gene
  IXK98_RS22900 (IXK98_22900) pilN 3077152..3077715 (-) 564 WP_171802179.1 PilN domain-containing protein Machinery gene
  IXK98_RS22905 (IXK98_22905) pilM 3077705..3078700 (-) 996 WP_069667325.1 type IV pilus assembly protein PilM Machinery gene
  IXK98_RS22910 (IXK98_22910) - 3078841..3081360 (+) 2520 WP_171383617.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22555.02 Da        Isoelectric Point: 9.0481

>NTDB_id=505405 IXK98_RS22895 WP_171802178.1 3076578..3077165(-) (pilO) [Vibrio europaeus strain 07/118 T2]
MYRFNLDIEEIGEWPLTYQLVVMAVLMAILQGGGYWLFAQPKLGQLDLLKQQEQQHKTSIALKTKKVVLLPRIKAQLDKL
SQRYDYLSRQLPAQKELASMLASVNQVGLQNSLTFTRIDWGHKHNQAFLYKLPLNIELTGDYHDIGDFSAAIASLPRIIN
FDDVYWHRVSLESSTLHFRVRAYTYQLKGEVKDES

Nucleotide


Download         Length: 588 bp        

>NTDB_id=505405 IXK98_RS22895 WP_171802178.1 3076578..3077165(-) (pilO) [Vibrio europaeus strain 07/118 T2]
GTGTATCGCTTTAACCTTGATATTGAAGAGATAGGCGAGTGGCCACTTACTTATCAGCTAGTCGTGATGGCCGTGTTGAT
GGCGATTTTACAGGGAGGCGGTTATTGGTTATTTGCTCAGCCTAAGCTTGGACAACTCGATCTTCTTAAACAGCAAGAGC
AGCAGCACAAAACCTCAATAGCATTGAAAACGAAAAAAGTGGTGTTGTTACCTAGAATAAAGGCCCAGCTTGATAAACTG
TCACAGCGTTATGATTACCTATCGCGTCAATTGCCCGCACAAAAAGAGCTTGCTTCTATGTTGGCGTCGGTTAATCAGGT
CGGCTTACAGAATTCCCTGACCTTTACCCGAATTGATTGGGGACACAAACACAACCAAGCGTTTCTATACAAGCTGCCAC
TCAACATTGAATTGACTGGCGATTATCACGATATCGGTGATTTTTCTGCAGCAATTGCCTCGCTACCGCGAATTATAAAT
TTTGATGATGTCTATTGGCATCGTGTCAGCTTAGAAAGCAGCACACTGCATTTTCGGGTCCGAGCTTACACCTATCAGCT
TAAAGGTGAGGTCAAAGATGAAAGTTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

66.842

97.436

0.651
  pilO Vibrio campbellii strain DS40M4

58.696

94.359

0.554