Detailed information NTG0505407

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IXK98_RS22905
• Genome accession: NZ_CP064858
• Coordinates: 3077705..3078700 (-)
• Length: 331 a.a.
• NCBI ID: WP_069667325.1
• Uniprot ID: A0A178JAY1
• Organism: Vibrio europaeus strain 07/118 T2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3072705..3083700

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IXK98_RS22880 (IXK98_22880)	aroK	3073682..3074200 (-)	519	WP_069667329.1	shikimate kinase AroK	-
IXK98_RS22885 (IXK98_22885)	pilQ	3074377..3076053 (-)	1677	WP_171802406.1	type IV pilus secretin PilQ	Machinery gene
IXK98_RS22890 (IXK98_22890)	pilP	3076085..3076588 (-)	504	WP_171802177.1	pilus assembly protein PilP	Machinery gene
IXK98_RS22895 (IXK98_22895)	pilO	3076578..3077165 (-)	588	WP_171802178.1	type 4a pilus biogenesis protein PilO	Machinery gene
IXK98_RS22900 (IXK98_22900)	pilN	3077152..3077715 (-)	564	WP_171802179.1	PilN domain-containing protein	Machinery gene
IXK98_RS22905 (IXK98_22905)	pilM	3077705..3078700 (-)	996	WP_069667325.1	type IV pilus assembly protein PilM	Machinery gene
IXK98_RS22910 (IXK98_22910)	-	3078841..3081360 (+)	2520	WP_171383617.1	penicillin-binding protein 1A	-
IXK98_RS22915 (IXK98_22915)	oxyR	3081423..3082316 (-)	894	WP_171802180.1	DNA-binding transcriptional regulator OxyR	-
IXK98_RS22920 (IXK98_22920)	-	3082489..3083220 (+)	732	WP_069667322.1	glutathione peroxidase	-
IXK98_RS22925 (IXK98_22925)	-	3083292..3083639 (-)	348	WP_004743292.1	RidA family protein	-

Sequence

Protein

• Length: 331 a.a.
• Molecular weight: 36405.82 Da
• Isoelectric Point: 7.2852

>NTDB_id=505407 IXK98_RS22905 WP_069667325.1 3077705..3078700(-) (pilM) [Vibrio europaeus strain 07/118 T2]
MGKSLVTGIEIGHHSIKAVVLKPNKGTVALVDYQELPIEAGIFSDNRMSNYQKIVKKLKELRKSLPRFSTKVALAIPDNA
VISKVLQIDSGLSDNEIEYAISDAFSHQTPFPIDDLYLDYVPLTSNGQSSPTHYQVYATKKELVAPRVEVFRSASFQPVV
VDLRAHALVHIWQLAARQQVKTDWLLIDVGFDHASLCMDLADKAPFCKEIVLATHEKEADSNHRLSLAQSREGFSAELLD
KIQRNLQLIVSMHGKSVNGIWLSGVGAAMPVLANEISTRLAIECELLNPLTLFSTTSQQPSANLLASGHRFTSAAGIALR
ALTWLESRNAA

Nucleotide

• Length: 996 bp

>NTDB_id=505407 IXK98_RS22905 WP_069667325.1 3077705..3078700(-) (pilM) [Vibrio europaeus strain 07/118 T2]
ATGGGTAAATCATTAGTTACAGGCATTGAAATTGGACACCACAGTATTAAAGCGGTGGTTCTCAAGCCAAACAAGGGCAC
AGTTGCTCTTGTCGACTATCAAGAATTGCCAATCGAAGCGGGCATTTTCTCTGATAACCGTATGTCAAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGGAAGAGTTTGCCACGATTTAGTACGAAAGTTGCATTAGCGATACCAGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGGGCTTAGCGACAATGAAATTGAGTACGCTATTTCCGATGCATTTTCCCA
TCAAACTCCGTTTCCAATTGACGATCTCTACTTAGATTATGTTCCCCTGACCAGTAATGGGCAGTCATCGCCAACTCACT
ACCAAGTATACGCGACGAAAAAAGAGCTAGTTGCCCCTAGAGTTGAAGTATTCAGAAGCGCTTCCTTTCAACCCGTAGTG
GTTGATTTACGTGCTCATGCTTTAGTGCATATTTGGCAGCTTGCTGCACGACAGCAGGTGAAAACCGATTGGCTTCTGAT
AGATGTTGGTTTTGACCACGCTTCGTTGTGTATGGACCTTGCTGATAAAGCGCCTTTTTGCAAAGAGATCGTTTTGGCAA
CCCATGAAAAAGAGGCGGATTCAAACCATCGCTTAAGCCTGGCGCAAAGCCGCGAAGGGTTCAGCGCCGAGTTACTAGAT
AAAATCCAACGCAATCTACAGCTTATTGTTTCTATGCACGGTAAGAGTGTTAACGGGATTTGGCTGTCAGGTGTTGGGGC
TGCCATGCCTGTTCTGGCGAATGAAATCAGCACGCGCCTTGCCATTGAATGTGAGCTTCTCAACCCGCTTACTCTTTTTT
CAACAACGAGCCAACAGCCATCCGCAAATCTGTTGGCTAGTGGGCATAGGTTCACATCGGCGGCTGGGATTGCTCTGCGC
GCGCTCACTTGGTTGGAGAGTCGCAATGCTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-195)

• Type IV pilus assembly protein PilM; (PilM_2) (234-321)

3D structure

• AlphaFold DB: A0A178JAY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	51.765	100	0.532
pilM	Vibrio campbellii strain DS40M4	41.964	100	0.426