Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IXY25_RS00295 Genome accession   NZ_CP064846
Coordinates   53398..54012 (+) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus velezensis strain BZR 86     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48398..59012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXY25_RS00260 (IXY25_00250) aroE 49085..49927 (+) 843 WP_025852995.1 shikimate dehydrogenase -
  IXY25_RS00265 (IXY25_00255) yhbY 49921..50211 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  IXY25_RS00270 (IXY25_00260) - 50222..50791 (+) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  IXY25_RS00275 (IXY25_00265) yqeK 50781..51362 (+) 582 WP_063174743.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IXY25_RS00280 (IXY25_00270) rsfS 51359..51715 (+) 357 WP_003152864.1 ribosome silencing factor -
  IXY25_RS00285 (IXY25_00275) - 51712..52449 (+) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  IXY25_RS00290 (IXY25_00280) comER 52518..53339 (-) 822 WP_014305449.1 late competence protein ComER -
  IXY25_RS00295 (IXY25_00285) comEA 53398..54012 (+) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  IXY25_RS00300 (IXY25_00290) - 54079..54648 (+) 570 WP_003152868.1 ComE operon protein 2 -
  IXY25_RS00305 (IXY25_00295) comEC 54649..56997 (+) 2349 WP_154303200.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IXY25_RS00310 (IXY25_00300) - 57016..57150 (-) 135 WP_003152870.1 YqzM family protein -
  IXY25_RS00315 (IXY25_00305) - 57191..57345 (+) 155 Protein_62 hypothetical protein -
  IXY25_RS00320 (IXY25_00310) holA 57385..58426 (+) 1042 Protein_63 DNA polymerase III subunit delta -
  IXY25_RS00325 (IXY25_00315) rpsT 58443..58709 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=505244 IXY25_RS00295 WP_015387983.1 53398..54012(+) (comEA) [Bacillus velezensis strain BZR 86]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=505244 IXY25_RS00295 WP_015387983.1 53398..54012(+) (comEA) [Bacillus velezensis strain BZR 86]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363