Detailed information NTG0505246

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: IXY25_RS00305
• Genome accession: NZ_CP064846
• Coordinates: 54649..56997 (+)
• Length: 782 a.a.
• NCBI ID: WP_154303200.1
• Uniprot ID: A0A6A8LFA2
• Organism: Bacillus velezensis strain BZR 86
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 49649..61997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IXY25_RS00265 (IXY25_00255)	yhbY	49921..50211 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
IXY25_RS00270 (IXY25_00260)	-	50222..50791 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
IXY25_RS00275 (IXY25_00265)	yqeK	50781..51362 (+)	582	WP_063174743.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IXY25_RS00280 (IXY25_00270)	rsfS	51359..51715 (+)	357	WP_003152864.1	ribosome silencing factor	-
IXY25_RS00285 (IXY25_00275)	-	51712..52449 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
IXY25_RS00290 (IXY25_00280)	comER	52518..53339 (-)	822	WP_014305449.1	late competence protein ComER	-
IXY25_RS00295 (IXY25_00285)	comEA	53398..54012 (+)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
IXY25_RS00300 (IXY25_00290)	-	54079..54648 (+)	570	WP_003152868.1	ComE operon protein 2	-
IXY25_RS00305 (IXY25_00295)	comEC	54649..56997 (+)	2349	WP_154303200.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IXY25_RS00310 (IXY25_00300)	-	57016..57150 (-)	135	WP_003152870.1	YqzM family protein	-
IXY25_RS00315 (IXY25_00305)	-	57191..57345 (+)	155	Protein_62	hypothetical protein	-
IXY25_RS00320 (IXY25_00310)	holA	57385..58426 (+)	1042	Protein_63	DNA polymerase III subunit delta	-
IXY25_RS00325 (IXY25_00315)	rpsT	58443..58709 (-)	267	WP_003152876.1	30S ribosomal protein S20	-
IXY25_RS00330 (IXY25_00320)	gpr	58912..60018 (+)	1107	WP_003152878.1	GPR endopeptidase	-
IXY25_RS00335 (IXY25_00325)	spoIIP	60086..61279 (+)	1194	WP_063174745.1	stage II sporulation protein P	-
IXY25_RS00340 (IXY25_00330)	-	61296..61634 (+)	339	WP_003152882.1	YqxA family protein	-

Sequence

Protein

• Length: 782 a.a.
• Molecular weight: 86622.59 Da
• Isoelectric Point: 9.1033

>NTDB_id=505246 IXY25_RS00305 WP_154303200.1 54649..56997(+) (comEC) [Bacillus velezensis strain BZR 86]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVIICLVSFCVYFFLYTVCDAVNVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRRIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNPY
LLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLASFPMNMVMVPFYTLFVIPVS
VIGFLLLLLSRQMGECLFNMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSAL
FFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFINAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGKG
VKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSPE
SSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGKE
NRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2349 bp

>NTDB_id=505246 IXY25_RS00305 WP_154303200.1 54649..56997(+) (comEC) [Bacillus velezensis strain BZR 86]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGGATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTATCATCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGTGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAAGAAAAGAGACGCATTGAACAGCTTGAACCGGGGATGCGCTGCACATTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATAACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATATTGCTGCTGTT
TTTACCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCGCCG
GAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCTTAC
CTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAAAGC
AGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATCATT
TTCAGCAAATTTCACTTGCCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTTTCT
GTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTAATATGTTTGACCTTGTGATGAAGCC
TGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCCTTC
TTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCTCTT
TTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATATCGG
GCAGGGAGACTCGCTGTTTATAAACGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTTATC
CCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAAGGG
GTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAATCA
TAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAGAAA
ACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCGGAG
TCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGACGGG
AGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTCATC
ACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAAGAG
AATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATATCAG
CGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCAGGG
CGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (507-718)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Competence protein (Competence) (208-468)

3D structure

• AlphaFold DB: A0A6A8LFA2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.088	98.721	0.554