Detailed information NTG0505189

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: IXY24_RS06245
• Genome accession: NZ_CP064845
• Coordinates: 1192163..1192777 (+)
• Length: 204 a.a.
• NCBI ID: WP_015387983.1
• Uniprot ID: A0A6A8LHQ3
• Organism: Bacillus velezensis strain BZR 277
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1187163..1197777

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IXY24_RS06210 (IXY24_06135)	aroE	1187850..1188692 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
IXY24_RS06215 (IXY24_06140)	yhbY	1188686..1188976 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
IXY24_RS06220 (IXY24_06145)	-	1188987..1189556 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
IXY24_RS06225 (IXY24_06150)	yqeK	1189546..1190127 (+)	582	WP_063174743.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IXY24_RS06230 (IXY24_06155)	rsfS	1190124..1190480 (+)	357	WP_003152864.1	ribosome silencing factor	-
IXY24_RS06235 (IXY24_06160)	-	1190477..1191214 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
IXY24_RS06240 (IXY24_06165)	comER	1191283..1192104 (-)	822	WP_014305449.1	late competence protein ComER	-
IXY24_RS06245 (IXY24_06170)	comEA	1192163..1192777 (+)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
IXY24_RS06250 (IXY24_06175)	-	1192844..1193413 (+)	570	WP_003152868.1	ComE operon protein 2	-
IXY24_RS06255 (IXY24_06180)	comEC	1193414..1195762 (+)	2349	WP_154303200.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IXY24_RS06260 (IXY24_06185)	-	1195781..1195915 (-)	135	WP_003152870.1	YqzM family protein	-
IXY24_RS06265 (IXY24_06190)	-	1195956..1196110 (+)	155	Protein_1222	hypothetical protein	-
IXY24_RS06270 (IXY24_06195)	holA	1196150..1197191 (+)	1042	Protein_1223	DNA polymerase III subunit delta	-
IXY24_RS06275 (IXY24_06200)	rpsT	1197208..1197474 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22255.41 Da
• Isoelectric Point: 5.5211

>NTDB_id=505189 IXY24_RS06245 WP_015387983.1 1192163..1192777(+) (comEA) [Bacillus velezensis strain BZR 277]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=505189 IXY24_RS06245 WP_015387983.1 1192163..1192777(+) (comEA) [Bacillus velezensis strain BZR 277]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A6A8LHQ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363