Detailed information NTG0505115

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLDRC3_RS11380
• Genome accession: NZ_CP064835
• Coordinates: 2214024..2214470 (-)
• Length: 148 a.a.
• NCBI ID: WP_031296844.1
• Uniprot ID: A0AAC9R3D6
• Organism: Lactococcus lactis subsp. lactis strain DRC3
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2209024..2219470

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLDRC3_RS11345 (LLDRC3_2176)	-	2209141..2209518 (-)	378	WP_063283703.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLDRC3_RS11350 (LLDRC3_2177)	-	2209723..2210589 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
LLDRC3_RS11355 (LLDRC3_2178)	-	2210628..2211437 (-)	810	WP_010906310.1	metal ABC transporter permease	-
LLDRC3_RS11360 (LLDRC3_2179)	-	2211430..2212167 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
LLDRC3_RS11365 (LLDRC3_2180)	-	2212344..2213186 (-)	843	WP_063283655.1	metal ABC transporter substrate-binding protein	-
LLDRC3_RS11370 (LLDRC3_2181)	-	2213183..2213620 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LLDRC3_RS11375 (LLDRC3_2182)	comGG	2213701..2213985 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLDRC3_RS11380 (LLDRC3_2183)	comGF	2214024..2214470 (-)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLDRC3_RS11385 (LLDRC3_2184)	comGE	2214433..2214729 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLDRC3_RS11390 (LLDRC3_2185)	comGD	2214701..2215099 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLDRC3_RS11395 (LLDRC3_2186)	comGC	2215092..2215475 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
LLDRC3_RS11400 (LLDRC3_2187)	comGB	2215489..2216562 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLDRC3_RS11405 (LLDRC3_2188)	comGA	2216456..2217394 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=505115 LLDRC3_RS11380 WP_031296844.1 2214024..2214470(-) (comGF) [Lactococcus lactis subsp. lactis strain DRC3]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=505115 LLDRC3_RS11380 WP_031296844.1 2214024..2214470(-) (comGF) [Lactococcus lactis subsp. lactis strain DRC3]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385