Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   KQ220_RS16380 Genome accession   NZ_CP076709
Coordinates   3390876..3392369 (-) Length   497 a.a.
NCBI ID   WP_216781221.1    Uniprot ID   -
Organism   Escherichia coli O158:H23 strain CFIAFB20140388     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3385876..3397369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ220_RS16350 (KQ220_16350) gspK 3386670..3387647 (-) 978 WP_034173461.1 type II secretion system minor pseudopilin GspK -
  KQ220_RS16355 (KQ220_16355) gspJ 3387644..3388249 (-) 606 WP_062875858.1 type II secretion system minor pseudopilin GspJ -
  KQ220_RS16360 (KQ220_16360) gspI 3388246..3388617 (-) 372 WP_112004728.1 type II secretion system minor pseudopilin GspI -
  KQ220_RS16365 (KQ220_16365) gspH 3388614..3389177 (-) 564 WP_112004730.1 type II secretion system minor pseudopilin GspH -
  KQ220_RS16370 (KQ220_16370) gspG 3389181..3389636 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  KQ220_RS16375 (KQ220_16375) gspF 3389653..3390876 (-) 1224 WP_097421248.1 type II secretion system inner membrane protein GspF -
  KQ220_RS16380 (KQ220_16380) pilB/pilB1 3390876..3392369 (-) 1494 WP_216781221.1 type II secretion system ATPase GspE Machinery gene
  KQ220_RS16385 (KQ220_16385) gspD 3392369..3394429 (-) 2061 WP_112004736.1 type II secretion system secretin GspD -
  KQ220_RS16390 (KQ220_16390) gspC 3394459..3395418 (-) 960 WP_112004738.1 type II secretion system protein GspC -
  KQ220_RS16395 (KQ220_16395) gspS2 3395436..3395846 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  KQ220_RS16400 (KQ220_16400) pilD 3395912..3396721 (-) 810 WP_216781222.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55187.37 Da        Isoelectric Point: 6.0074

>NTDB_id=504320 KQ220_RS16380 WP_216781221.1 3390876..3392369(-) (pilB/pilB1) [Escherichia coli O158:H23 strain CFIAFB20140388]
MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQALPA
TDEEKRLLGIIDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=504320 KQ220_RS16380 WP_216781221.1 3390876..3392369(-) (pilB/pilB1) [Escherichia coli O158:H23 strain CFIAFB20140388]
ATGGTGCCTGTAGCACAGGAAACCTCCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGTTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCAGCAACTGACC
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCTGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTATCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.059

85.513

0.402
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362