Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IVG45_RS19515 Genome accession   NZ_CP064653
Coordinates   4164035..4165189 (+) Length   384 a.a.
NCBI ID   WP_196435430.1    Uniprot ID   A0A7T0JM75
Organism   Methylomonas sp. LL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4159035..4170189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IVG45_RS19485 (IVG45_19440) zapD 4159431..4160201 (+) 771 WP_196435425.1 cell division protein ZapD -
  IVG45_RS19490 (IVG45_19445) yacG 4160201..4160404 (+) 204 WP_196435426.1 DNA gyrase inhibitor YacG -
  IVG45_RS19495 (IVG45_19450) - 4160419..4161351 (-) 933 WP_196435427.1 Nudix family hydrolase -
  IVG45_RS19500 (IVG45_19455) argJ 4161352..4162566 (-) 1215 WP_196435428.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  IVG45_RS19505 (IVG45_19460) - 4162588..4162863 (-) 276 WP_230874663.1 sulfurtransferase TusA family protein -
  IVG45_RS19510 (IVG45_19465) - 4162860..4163903 (-) 1044 WP_196435429.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  IVG45_RS19515 (IVG45_19470) pilU 4164035..4165189 (+) 1155 WP_196435430.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IVG45_RS19520 (IVG45_19475) - 4165234..4165557 (-) 324 WP_196435431.1 NGG1p interacting factor NIF3 -
  IVG45_RS19525 (IVG45_19480) - 4165568..4165720 (-) 153 WP_196435432.1 hypothetical protein -
  IVG45_RS19530 (IVG45_19485) - 4165870..4166769 (+) 900 WP_196434642.1 IS1595 family transposase -
  IVG45_RS19535 (IVG45_19490) - 4166942..4167985 (+) 1044 WP_196435433.1 LysM peptidoglycan-binding domain-containing protein -
  IVG45_RS19540 (IVG45_19495) dprA 4168047..4169144 (+) 1098 WP_230874664.1 DNA-processing protein DprA -
  IVG45_RS19545 (IVG45_19500) - 4169153..4169620 (+) 468 WP_196435434.1 DUF494 domain-containing protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42310.22 Da        Isoelectric Point: 6.0967

>NTDB_id=504058 IVG45_RS19515 WP_196435430.1 4164035..4165189(+) (pilU) [Methylomonas sp. LL1]
MEFKDYLKILVQHDGSDLYLTVGAPPAAKFQGSLKPIENVKLTNERLKEIANSIMDNDQRSNFEHVPEMNLAISEPGIGR
FRVNIFKQRNSYALVIRNIKVDIPNADALGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLQAFISQRLVPTVEGKRVAAIEVLLGTKLVSDLIQKGEVHAIKEAMEKSENIGMQTFDSHL
LKLYKSGVISLEEALRNSDSPNNLKLKINLSEGLGSATASTGSGLDKLSLEAISKDGDDAEEGH

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=504058 IVG45_RS19515 WP_196435430.1 4164035..4165189(+) (pilU) [Methylomonas sp. LL1]
ATGGAATTCAAGGATTATCTAAAAATATTGGTTCAACACGATGGTTCAGACCTGTATTTGACGGTAGGAGCTCCGCCGGC
GGCAAAATTTCAGGGTAGCTTGAAGCCTATCGAAAACGTCAAGTTGACCAACGAGCGTCTGAAGGAAATCGCCAACAGCA
TCATGGATAACGATCAGCGCAGCAACTTCGAACATGTGCCGGAAATGAATCTGGCGATTTCCGAGCCGGGTATAGGCCGT
TTTAGGGTCAATATTTTCAAACAACGTAACTCTTATGCTCTGGTGATTCGCAACATCAAGGTCGATATTCCCAATGCCGA
TGCCTTGGGCTTGCCGCAAATCCTTAAAGACAAAATTATGGAAAAGCGCGGCCTAATTCTGTTCGTCGGCGGTACCGGTT
CGGGAAAGTCCACGTCGTTGGCGGCTTTGATCGATCACCGCAATAGCAATTCTTCCGGCCACATCATCACCATCGAAGAT
CCGATCGAATATGTACACCCGCATAAAAAATCGCTGGTCAATCAGCGCGAAGTGGGAGTCGATACATTGAGTTACGAGGA
TGCGCTGAAAAACACCTTGCGCCAAGCCCCCGACGTGATTCTGATCGGCGAAATCCGCAGCCAGGAAACCATGGAGCATG
CGCTGGCTTTTGCCGAAACCGGCCATTTATGTCTATCGACGCTACATGCCAACAATGCCAATCAGGCTTTGGATAGGATC
ATCAATTTCTTTCCGGAAGAGCGCCGTAATCAGTTGCTGCTGGATTTATCGCTGAATCTGCAGGCCTTCATTTCGCAACG
CCTGGTGCCGACCGTCGAAGGCAAGCGGGTGGCTGCGATCGAGGTTTTGCTGGGCACCAAGCTGGTTAGCGATTTGATTC
AGAAGGGCGAGGTGCATGCCATCAAGGAGGCGATGGAAAAGTCCGAGAACATCGGCATGCAGACTTTCGACAGCCATTTG
TTGAAGCTTTACAAAAGCGGCGTGATTTCATTGGAGGAGGCCTTGCGTAATTCCGATTCCCCTAACAACCTGAAATTAAA
AATCAATCTGAGCGAAGGTCTCGGTTCGGCCACGGCATCCACGGGAAGCGGATTGGATAAGCTGTCTTTGGAAGCGATCA
GCAAGGATGGAGATGATGCGGAGGAAGGGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0JM75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.948

95.573

0.544
  pilU Acinetobacter baylyi ADP1

54.52

92.188

0.503
  pilU Vibrio cholerae strain A1552

49.718

92.188

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.331

94.271

0.38