Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   KP725_RS10370 Genome accession   NZ_CP076645
Coordinates   2132328..2133338 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain MSI001     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2127328..2138338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP725_RS10340 (KP725_10340) nudB 2129052..2129504 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  KP725_RS10345 (KP725_10345) yebC 2129533..2130273 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  KP725_RS10350 (KP725_10350) ruvC 2130308..2130829 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  KP725_RS10355 (KP725_10355) yebB 2130831..2131433 (-) 603 WP_163454920.1 YebB family permuted papain-like enzyme -
  KP725_RS10360 (KP725_10360) yobI 2131504..2131569 (+) 66 WP_072094247.1 stress response small protein YobI -
  KP725_RS10365 (KP725_10365) ruvA 2131708..2132319 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  KP725_RS10370 (KP725_10370) ruvB 2132328..2133338 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  KP725_RS10375 (KP725_10375) znuB 2133485..2134270 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  KP725_RS10380 (KP725_10380) znuC 2134267..2135022 (-) 756 WP_047082488.1 zinc ABC transporter ATP-binding protein ZnuC -
  KP725_RS10385 (KP725_10385) znuA 2135101..2136033 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  KP725_RS10390 (KP725_10390) mepM 2136049..2137371 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=503326 KP725_RS10370 WP_000568519.1 2132328..2133338(+) (ruvB) [Escherichia coli strain MSI001]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=503326 KP725_RS10370 WP_000568519.1 2132328..2133338(+) (ruvB) [Escherichia coli strain MSI001]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494