Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   I14_RS18430 Genome accession   NC_017652
Coordinates   3734861..3735985 (-) Length   374 a.a.
NCBI ID   WP_000228508.1    Uniprot ID   A0A0D8VMA7
Organism   Escherichia coli str. 'clone D i14'     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3729861..3740985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I14_RS18400 (i14_3722) yrdA 3731661..3732215 (+) 555 WP_001286206.1 gamma carbonic anhydrase family protein -
  I14_RS18405 (i14_3723) yrdB 3732191..3732448 (-) 258 WP_001070581.1 DUF1488 domain-containing protein -
  I14_RS18410 (i14_3724) aroE 3732445..3733263 (-) 819 WP_000451246.1 shikimate dehydrogenase -
  I14_RS18415 (i14_3725) tsaC 3733268..3733840 (-) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  I14_RS18420 (i14_3726) yrdD 3733845..3734387 (-) 543 WP_001312137.1 type I DNA topoisomerase -
  I14_RS18425 (i14_3727) smg 3734416..3734889 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  I14_RS18430 (i14_3728) dprA 3734861..3735985 (-) 1125 WP_000228508.1 DNA-protecting protein DprA Machinery gene
  I14_RS18435 (i14_3729) def 3736115..3736624 (+) 510 WP_000114984.1 peptide deformylase -
  I14_RS18440 (i14_3730) fmt 3736639..3737586 (+) 948 WP_000004425.1 methionyl-tRNA formyltransferase -
  I14_RS18445 (i14_3731) rsmB 3737638..3738927 (+) 1290 WP_000744773.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  I14_RS18450 (i14_3732) trkA 3738949..3740325 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  I14_RS18455 (i14_3733) mscL 3740455..3740868 (+) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40892.82 Da        Isoelectric Point: 6.0395

>NTDB_id=50163 I14_RS18430 WP_000228508.1 3734861..3735985(-) (dprA) [Escherichia coli str. 'clone D i14']
MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=50163 I14_RS18430 WP_000228508.1 3734861..3735985(-) (dprA) [Escherichia coli str. 'clone D i14']
ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment