Detailed information NTG0500832

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IUD20_RS06240
• Genome accession: NZ_CP064334
• Coordinates: 1370806..1371936 (+)
• Length: 376 a.a.
• NCBI ID: WP_165804224.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1365806..1376936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUD20_RS06220 (IUD20_06220)	-	1366093..1366917 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
IUD20_RS06225 (IUD20_06225)	-	1367551..1368378 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
IUD20_RS06230 (IUD20_06230)	-	1368736..1368948 (-)	213	WP_004087301.1	hypothetical protein	-
IUD20_RS06235 (IUD20_06235)	pilT	1369606..1370640 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
IUD20_RS06240 (IUD20_06240)	pilU	1370806..1371936 (+)	1131	WP_165804224.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IUD20_RS06245 (IUD20_06245)	-	1372629..1373597 (-)	969	WP_004087304.1	YafY family protein	-
IUD20_RS06250 (IUD20_06250)	-	1374446..1375255 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
IUD20_RS06255 (IUD20_06255)	hemC	1375309..1376226 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42426.68 Da
• Isoelectric Point: 6.4480

>NTDB_id=500832 IUD20_RS06240 WP_165804224.1 1370806..1371936(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFCVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=500832 IUD20_RS06240 WP_165804224.1 1370806..1371936(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-8]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCTGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	50.562	94.681	0.479
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399