Detailed information NTG0500781

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IUD25_RS06090
• Genome accession: NZ_CP064328
• Coordinates: 1353892..1355022 (+)
• Length: 376 a.a.
• NCBI ID: WP_194957378.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1348892..1360022

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUD25_RS06070 (IUD25_06070)	-	1349179..1350003 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
IUD25_RS06075 (IUD25_06075)	-	1350637..1351464 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
IUD25_RS06080 (IUD25_06080)	-	1351822..1352034 (-)	213	WP_004087301.1	hypothetical protein	-
IUD25_RS06085 (IUD25_06085)	pilT	1352692..1353726 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
IUD25_RS06090 (IUD25_06090)	pilU	1353892..1355022 (+)	1131	WP_194957378.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IUD25_RS06095 (IUD25_06095)	-	1355715..1356683 (-)	969	WP_004087304.1	YafY family protein	-
IUD25_RS06100 (IUD25_06100)	-	1357532..1358341 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
IUD25_RS06105 (IUD25_06105)	hemC	1358395..1359312 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42452.71 Da
• Isoelectric Point: 6.5563

>NTDB_id=500781 IUD25_RS06090 WP_194957378.1 1353892..1355022(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-14]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECSFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=500781 IUD25_RS06090 WP_194957378.1 1353892..1355022(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain Bakersfield-14]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAGCTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	50.562	94.681	0.479
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.713	96.277	0.402