Detailed information NTG0500748

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IUD24_RS05840
• Genome accession: NZ_CP064325
• Coordinates: 1322354..1323484 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1317354..1328484

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUD24_RS05820 (IUD24_05820)	-	1317678..1318502 (+)	825	WP_021358442.1	glycosyltransferase family 2 protein	-
IUD24_RS05825 (IUD24_05825)	-	1319119..1319946 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
IUD24_RS05830 (IUD24_05830)	-	1320331..1320543 (-)	213	WP_021358439.1	hypothetical protein	-
IUD24_RS05835 (IUD24_05835)	pilT	1321165..1322199 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
IUD24_RS05840 (IUD24_05840)	pilU	1322354..1323484 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IUD24_RS05845 (IUD24_05845)	-	1324153..1325121 (-)	969	WP_004084952.1	YafY family protein	-
IUD24_RS05850 (IUD24_05850)	-	1325904..1326713 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
IUD24_RS05855 (IUD24_05855)	hemC	1326767..1327684 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
IUD24_RS05860 (IUD24_05860)	-	1327692..1328450 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=500748 IUD24_RS05840 WP_004084950.1 1322354..1323484(+) (pilU) [Xylella fastidiosa subsp. multiplex strain Red Oak]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=500748 IUD24_RS05840 WP_004084950.1 1322354..1323484(+) (pilU) [Xylella fastidiosa subsp. multiplex strain Red Oak]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402