Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRG857_RS14820 Genome accession   NC_017634
Coordinates   3068226..3069206 (-) Length   326 a.a.
NCBI ID   WP_001683272.1    Uniprot ID   A0A0H3EPC5
Organism   Escherichia coli O83:H1 str. NRG 857C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063226..3074206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG857_RS14790 (NRG857_14460) yggI 3063891..3064388 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  NRG857_RS14795 (NRG857_14465) endA 3064483..3065190 (+) 708 WP_001327408.1 deoxyribonuclease I -
  NRG857_RS14800 (NRG857_14470) rsmE 3065270..3066001 (+) 732 WP_014640203.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NRG857_RS14805 (NRG857_14475) gshB 3066014..3066964 (+) 951 WP_000593260.1 glutathione synthase -
  NRG857_RS14810 (NRG857_14480) yqgE 3067073..3067636 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  NRG857_RS14815 (NRG857_14485) ruvX 3067636..3068052 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  NRG857_RS14820 (NRG857_14490) pilT 3068226..3069206 (-) 981 WP_001683272.1 type IV pilus twitching motility protein PilT Machinery gene
  NRG857_RS14825 (NRG857_14495) yggS 3069224..3069928 (+) 705 WP_000997805.1 pyridoxal phosphate homeostasis protein -
  NRG857_RS14830 (NRG857_14500) yggT 3069946..3070512 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NRG857_RS14835 (NRG857_14505) yggU 3070509..3070799 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  NRG857_RS14840 (NRG857_14510) rdgB 3070807..3071400 (+) 594 WP_001174737.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  NRG857_RS14845 (NRG857_14515) hemW 3071393..3072529 (+) 1137 WP_000239976.1 radical SAM family heme chaperone HemW -
  NRG857_RS14850 (NRG857_14520) yggM 3072594..3073601 (-) 1008 WP_000745184.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35972.15 Da        Isoelectric Point: 5.7980

>NTDB_id=50028 NRG857_RS14820 WP_001683272.1 3068226..3069206(-) (pilT) [Escherichia coli O83:H1 str. NRG 857C]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=50028 NRG857_RS14820 WP_001683272.1 3068226..3069206(-) (pilT) [Escherichia coli O83:H1 str. NRG 857C]
ATGAATATGGAAGAAATTGTAGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EPC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365

Multiple sequence alignment