Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   IRJ27_RS12925 Genome accession   NZ_CP064095
Coordinates   2531417..2532808 (+) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis strain N1303-2Ay     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2526417..2537808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ27_RS12905 yvyE 2527569..2528222 (-) 654 WP_003227979.1 YigZ family protein -
  IRJ27_RS12910 degS 2528439..2529596 (+) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  IRJ27_RS12915 degU 2529679..2530368 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  IRJ27_RS12920 fakBA 2530466..2531311 (+) 846 WP_003244125.1 DegV family protein -
  IRJ27_RS12925 comFA 2531417..2532808 (+) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  IRJ27_RS12930 comFB 2532868..2533164 (+) 297 WP_003227989.1 late competence protein ComFB -
  IRJ27_RS12935 comFC 2533128..2533850 (+) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  IRJ27_RS12940 yvyF 2533924..2534343 (+) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  IRJ27_RS12945 flgM 2534424..2534690 (+) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  IRJ27_RS12950 flgN 2534706..2535188 (+) 483 WP_003227999.1 flagellar protein FlgN -
  IRJ27_RS12955 flgK 2535207..2536730 (+) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  IRJ27_RS12960 flgL 2536741..2537637 (+) 897 WP_003228004.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=499405 IRJ27_RS12925 WP_003243962.1 2531417..2532808(+) (comFA) [Bacillus subtilis strain N1303-2Ay]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=499405 IRJ27_RS12925 WP_003243962.1 2531417..2532808(+) (comFA) [Bacillus subtilis strain N1303-2Ay]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1