Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   IRJ27_RS10035 Genome accession   NZ_CP064095
Coordinates   1975544..1976062 (+) Length   172 a.a.
NCBI ID   WP_003219228.1    Uniprot ID   A0A063XE16
Organism   Bacillus subtilis strain N1303-2Ay     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 1970544..1981062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ27_RS10015 yyzM 1971618..1971824 (+) 207 WP_003226838.1 DUF951 family protein -
  IRJ27_RS10020 yyaE 1971875..1973878 (+) 2004 WP_003244290.1 molybdopterin-dependent oxidoreductase -
  IRJ27_RS10025 ychF 1974005..1975105 (+) 1101 WP_003242727.1 redox-regulated ATPase YchF -
  IRJ27_RS10030 rpsF 1975216..1975503 (+) 288 WP_003244064.1 30S ribosomal protein S6 -
  IRJ27_RS10035 ssbA 1975544..1976062 (+) 519 WP_003219228.1 single-stranded DNA-binding protein SsbA Machinery gene
  IRJ27_RS10040 rpsR 1976106..1976345 (+) 240 WP_003219224.1 30S ribosomal protein S18 -
  IRJ27_RS10045 xth 1976410..1977168 (+) 759 WP_003243194.1 exodeoxyribonuclease III -
  IRJ27_RS10050 ccpB 1977227..1978162 (+) 936 WP_003243573.1 transcriptional regulator CcpB -
  IRJ27_RS10055 yyaH 1978218..1978598 (+) 381 WP_003244460.1 VOC family protein -
  IRJ27_RS10060 maa 1978616..1979170 (+) 555 WP_003242928.1 sugar O-acetyltransferase -
  IRJ27_RS10065 yyaJ 1979204..1980559 (-) 1356 WP_003243011.1 MFS transporter -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 18742.31 Da        Isoelectric Point: 4.7621

>NTDB_id=499395 IRJ27_RS10035 WP_003219228.1 1975544..1976062(+) (ssbA) [Bacillus subtilis strain N1303-2Ay]
MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNGGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=499395 IRJ27_RS10035 WP_003219228.1 1975544..1976062(+) (ssbA) [Bacillus subtilis strain N1303-2Ay]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAACGGTGCGGCTGTTGC
TACGTTTACTCTTGCTGTGAATCGTACATTTACGAACCAGTCCGGAGAACGTGAGGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTCTTGAAAAAAGGAAGCCTTGCAGGCGTAGATGGCCGTTTACAAACAAGA
AACTATGAAAACCAGCAAGGACAGCGTGTCTTCGTGACAGAGGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCGAAAAA
CGGCGGCGGTTCTGGTTCAGGTGGATACAACGAAGGAAACAGCGGCGGAGGCCAGTACTTTGGCGGAGGCCAAAATGATA
ATCCATTTGGGGGAAATCAAAACAACCAGAGACGCAATCAGGGGAACAGCTTTAATGATGACCCATTTGCCAACGACGGC
AAACCGATTGACATCTCGGATGATGATCTTCCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A063XE16

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ssb Latilactobacillus sakei subsp. sakei 23K

58.192

100

0.599
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395
  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378