Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   IRB21_RS14100 Genome accession   NZ_CP064089
Coordinates   2722455..2722748 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus altitudinis strain N435-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2717455..2727748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRB21_RS14055 sinR 2717625..2717960 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  IRB21_RS14060 - 2718028..2718828 (-) 801 WP_008342195.1 TasA family protein -
  IRB21_RS14065 - 2718887..2719459 (-) 573 WP_017357979.1 signal peptidase I -
  IRB21_RS14070 tapA 2719509..2720036 (-) 528 WP_073413951.1 amyloid fiber anchoring/assembly protein TapA -
  IRB21_RS14075 - 2720289..2720615 (+) 327 WP_242730660.1 DUF3889 domain-containing protein -
  IRB21_RS14080 - 2720650..2720838 (-) 189 WP_008360237.1 YqzE family protein -
  IRB21_RS14085 comGG 2720899..2721282 (-) 384 WP_242730662.1 competence type IV pilus minor pilin ComGG -
  IRB21_RS14090 comGF 2721279..2721776 (-) 498 WP_242730664.1 competence type IV pilus minor pilin ComGF -
  IRB21_RS20625 comGE 2721718..2722032 (-) 315 WP_341275286.1 competence type IV pilus minor pilin ComGE -
  IRB21_RS14095 comGD 2722016..2722462 (-) 447 WP_203085332.1 competence type IV pilus minor pilin ComGD -
  IRB21_RS14100 comGC 2722455..2722748 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  IRB21_RS14105 comGB 2722765..2723805 (-) 1041 WP_242730666.1 competence type IV pilus assembly protein ComGB -
  IRB21_RS14110 comGA 2723786..2724856 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  IRB21_RS14115 - 2725314..2726186 (-) 873 WP_046527023.1 STAS domain-containing protein -
  IRB21_RS14125 - 2726412..2726792 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IRB21_RS14130 - 2727005..2727247 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=498970 IRB21_RS14100 WP_008342186.1 2722455..2722748(-) (comGC) [Bacillus altitudinis strain N435-3]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=498970 IRB21_RS14100 WP_008342186.1 2722455..2722748(-) (comGC) [Bacillus altitudinis strain N435-3]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCGAATGGAAAGGCAATTTTTATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392