Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IRB21_RS14110 Genome accession   NZ_CP064089
Coordinates   2723786..2724856 (-) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain N435-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718786..2729856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRB21_RS14065 - 2718887..2719459 (-) 573 WP_017357979.1 signal peptidase I -
  IRB21_RS14070 tapA 2719509..2720036 (-) 528 WP_073413951.1 amyloid fiber anchoring/assembly protein TapA -
  IRB21_RS14075 - 2720289..2720615 (+) 327 WP_242730660.1 DUF3889 domain-containing protein -
  IRB21_RS14080 - 2720650..2720838 (-) 189 WP_008360237.1 YqzE family protein -
  IRB21_RS14085 comGG 2720899..2721282 (-) 384 WP_242730662.1 competence type IV pilus minor pilin ComGG -
  IRB21_RS14090 comGF 2721279..2721776 (-) 498 WP_242730664.1 competence type IV pilus minor pilin ComGF -
  IRB21_RS20625 comGE 2721718..2722032 (-) 315 WP_341275286.1 competence type IV pilus minor pilin ComGE -
  IRB21_RS14095 comGD 2722016..2722462 (-) 447 WP_203085332.1 competence type IV pilus minor pilin ComGD -
  IRB21_RS14100 comGC 2722455..2722748 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  IRB21_RS14105 comGB 2722765..2723805 (-) 1041 WP_242730666.1 competence type IV pilus assembly protein ComGB -
  IRB21_RS14110 comGA 2723786..2724856 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  IRB21_RS14115 - 2725314..2726186 (-) 873 WP_046527023.1 STAS domain-containing protein -
  IRB21_RS14125 - 2726412..2726792 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IRB21_RS14130 - 2727005..2727247 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  IRB21_RS14135 - 2727296..2727934 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  IRB21_RS14140 - 2728095..2728268 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  IRB21_RS14145 - 2728293..2728607 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=498971 IRB21_RS14110 WP_017357987.1 2723786..2724856(-) (comGA) [Bacillus altitudinis strain N435-3]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=498971 IRB21_RS14110 WP_017357987.1 2723786..2724856(-) (comGA) [Bacillus altitudinis strain N435-3]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAGGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTACAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGAGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649