Detailed information NTG0498223

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: IGI24_RS10010
• Genome accession: NZ_CP075472
• Coordinates: 1928164..1929174 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain NT1F25
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1923164..1934174

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IGI24_RS09980	nudB	1924888..1925340 (+)	453	WP_032141314.1	dihydroneopterin triphosphate diphosphatase	-
IGI24_RS09985	yebC	1925369..1926109 (+)	741	WP_097502008.1	YebC/PmpR family DNA-binding transcriptional regulator	-
IGI24_RS09990	ruvC	1926144..1926665 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
IGI24_RS09995	yebB	1926667..1927269 (-)	603	WP_001024904.1	YebB family permuted papain-like enzyme	-
IGI24_RS10000	yobI	1927340..1927405 (+)	66	WP_072094247.1	stress response small protein YobI	-
IGI24_RS10005	ruvA	1927544..1928155 (+)	612	WP_000580327.1	Holliday junction branch migration protein RuvA	-
IGI24_RS10010	ruvB	1928164..1929174 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
IGI24_RS10015	znuB	1929515..1930300 (-)	786	WP_000571480.1	zinc ABC transporter permease subunit ZnuB	-
IGI24_RS10020	znuC	1930297..1931052 (-)	756	WP_000202990.1	zinc ABC transporter ATP-binding protein ZnuC	-
IGI24_RS10025	znuA	1931131..1932063 (+)	933	WP_001300644.1	zinc ABC transporter substrate-binding protein ZnuA	-
IGI24_RS10030	mepM	1932079..1933401 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=498223 IGI24_RS10010 WP_000568519.1 1928164..1929174(+) (ruvB) [Escherichia coli strain NT1F25]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=498223 IGI24_RS10010 WP_000568519.1 1928164..1929174(+) (ruvB) [Escherichia coli strain NT1F25]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGGATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGGGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494