Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IRI77_RS27545 Genome accession   NZ_CP063849
Coordinates   6976965..6978602 (+) Length   545 a.a.
NCBI ID   WP_194448193.1    Uniprot ID   A0A7S7NMZ7
Organism   Paludibaculum fermentans strain P105     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6971965..6983602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRI77_RS27520 (IRI77_27520) - 6973163..6973672 (-) 510 WP_194448188.1 DinB family protein -
  IRI77_RS27525 (IRI77_27525) - 6973753..6974706 (+) 954 WP_194448189.1 YafY family protein -
  IRI77_RS27530 (IRI77_27530) - 6975244..6975801 (+) 558 WP_194448190.1 RNA polymerase sigma factor -
  IRI77_RS27535 (IRI77_27535) - 6975827..6976423 (+) 597 WP_194448191.1 zf-HC2 domain-containing protein -
  IRI77_RS27540 (IRI77_27540) - 6976423..6976962 (+) 540 WP_194448192.1 hypothetical protein -
  IRI77_RS27545 (IRI77_27545) pilF 6976965..6978602 (+) 1638 WP_194448193.1 type II secretion system ATPase GspE Machinery gene
  IRI77_RS27550 (IRI77_27550) - 6978612..6979820 (+) 1209 WP_194448194.1 type II secretion system F family protein -
  IRI77_RS27555 (IRI77_27555) gspG 6979857..6980303 (+) 447 WP_194448195.1 type II secretion system major pseudopilin GspG -
  IRI77_RS27560 (IRI77_27560) - 6980309..6980803 (+) 495 WP_194448196.1 Tfp pilus assembly protein FimT/FimU -
  IRI77_RS27565 (IRI77_27565) - 6980808..6981278 (+) 471 WP_194448197.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IRI77_RS27570 (IRI77_27570) - 6981275..6982006 (+) 732 WP_194448198.1 type II secretion system protein J -
  IRI77_RS27575 (IRI77_27575) - 6981996..6983009 (+) 1014 WP_194448199.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 545 a.a.        Molecular weight: 60629.64 Da        Isoelectric Point: 5.4627

>NTDB_id=497077 IRI77_RS27545 WP_194448193.1 6976965..6978602(+) (pilF) [Paludibaculum fermentans strain P105]
MRLGEILIQRGQLAQEELDRALEMQKERGDKLGRILVDLGYVAQRDILAALSEQLNVAIAKLDGPPLVSPETERLSARFL
RQAKALPLRMDGGALILAVADPLDFETLAAAKSATGLRIRPELAMESEIVDAIDKFYGEEEKQDAAFAQDGEENAADLEH
LRDMASEAPVIRLVNAMIAQAVEKRGSDIHIEPFEKEFRVRFRIDGVLHDQEAPPRELKAAVISRLKLMAKLNIAERRLP
QDGRIKIKTMGREVDLRVSTLPTLYGESVVMRLLDRSAGDFYDLLNLGFDDHMLRRMEHFTALPHGIFLVTGPTGSGKST
TLYSALKRINQSDKKIITIEDPVEYQMDGINQIHVNTQIGLTFAMGLRHIVRQDPDVIMVGEIRDRETADVAIRSALTGH
FVYSSLHTNDAPSAVTRLTDMGVENYLICSSLVAVLAQRLVRVVCPGCRQEAGERLTPLGDTIPTFRGTGCERCFGSGYK
GRVGIFELMEMNDELRKLVMRNADAAEITQAARSNGMRNLREDGWMKVANGVTTADEVLRVTQEF

Nucleotide


Download         Length: 1638 bp        

>NTDB_id=497077 IRI77_RS27545 WP_194448193.1 6976965..6978602(+) (pilF) [Paludibaculum fermentans strain P105]
ATGCGGCTGGGTGAAATACTGATACAGCGCGGGCAACTGGCGCAGGAAGAGTTGGACCGCGCGCTGGAGATGCAGAAGGA
GCGCGGCGACAAACTGGGCCGCATCCTGGTAGATCTCGGGTACGTTGCCCAGCGGGACATTCTCGCGGCGCTTTCCGAGC
AGTTGAACGTCGCGATTGCGAAACTGGATGGCCCGCCGCTGGTCTCCCCCGAGACGGAGCGGCTGTCGGCCCGCTTCCTG
CGGCAAGCCAAGGCCCTGCCATTGCGCATGGACGGCGGAGCGCTGATCCTGGCTGTGGCCGATCCGCTGGACTTCGAGAC
GCTGGCCGCCGCCAAGTCGGCCACCGGGCTGCGCATCCGGCCCGAGCTCGCCATGGAGTCGGAGATCGTCGATGCGATCG
ATAAGTTCTACGGCGAGGAAGAGAAGCAGGACGCCGCGTTTGCGCAGGATGGCGAGGAGAATGCCGCCGACCTGGAGCAT
CTGCGGGACATGGCCAGCGAGGCGCCGGTCATCCGCCTGGTGAACGCCATGATCGCCCAGGCCGTGGAGAAGCGCGGCAG
CGACATTCACATCGAGCCGTTTGAGAAAGAGTTCCGCGTCCGGTTCCGGATTGACGGTGTGCTGCACGACCAGGAGGCTC
CGCCGCGGGAGCTGAAGGCGGCCGTGATTTCGCGCCTGAAACTGATGGCGAAGCTGAACATCGCGGAACGGCGCCTGCCG
CAGGACGGCCGTATCAAGATCAAGACGATGGGCCGCGAGGTCGATTTGCGCGTGTCCACCTTGCCGACGCTCTACGGCGA
GAGTGTCGTGATGCGCCTGCTGGACCGTAGCGCCGGCGACTTCTACGACCTGCTGAATCTCGGCTTCGACGACCACATGC
TGCGCCGCATGGAGCACTTCACCGCTCTGCCGCACGGCATTTTCCTGGTGACCGGCCCCACCGGCAGCGGCAAGTCGACC
ACGTTGTATTCGGCGCTGAAGCGGATCAACCAATCGGACAAGAAGATCATCACCATCGAGGATCCGGTGGAATACCAGAT
GGACGGTATCAACCAGATCCACGTGAATACGCAGATCGGGCTTACGTTCGCGATGGGGCTGCGGCACATCGTGCGCCAGG
ATCCGGACGTGATCATGGTGGGCGAAATCCGCGACCGCGAGACGGCGGATGTCGCCATCCGCTCCGCTTTGACCGGCCAC
TTCGTCTACTCCTCGCTGCATACGAACGACGCGCCCAGCGCCGTCACCCGCCTCACGGATATGGGGGTGGAGAATTACCT
GATCTGTTCGTCGCTGGTGGCTGTGCTGGCCCAGCGCCTGGTGCGGGTGGTCTGTCCGGGCTGCCGCCAGGAGGCGGGCG
AGAGGCTGACGCCGTTGGGCGATACGATCCCCACATTTCGAGGAACGGGCTGCGAGCGCTGCTTCGGCTCCGGCTACAAA
GGCCGGGTGGGCATCTTCGAACTGATGGAGATGAACGATGAGCTGCGCAAGCTGGTGATGCGCAATGCGGATGCGGCCGA
GATCACGCAGGCCGCGCGTTCCAACGGTATGCGCAACCTGCGCGAGGATGGCTGGATGAAGGTGGCCAACGGCGTCACCA
CGGCCGATGAGGTGCTGCGTGTCACCCAGGAGTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-142)

Type II/IV secretion system protein (T2SSE)

(176-443)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S7NMZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

44.257

100

0.481
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.393

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

37.886

100

0.428
  pilB Vibrio campbellii strain DS40M4

40.036

100

0.413
  pilB Vibrio parahaemolyticus RIMD 2210633

39.432

100

0.407
  pilB Acinetobacter baylyi ADP1

36.655

100

0.378
  pilB Legionella pneumophila strain ERS1305867

38.433

98.349

0.378
  pilB Vibrio cholerae strain A1552

42.292

88.073

0.372