Detailed information NTG0497061

Bioinformatically predicted

Overview

• Name: pilB/pilB1
• Type: Machinery gene
• Locus tag: ISF26_RS09955
• Genome accession: NZ_CP063845
• Coordinates: 2039046..2040671 (+)
• Length: 541 a.a.
• NCBI ID: WP_230843732.1
• Uniprot ID: -
• Organism: Gloeobacter morelensis MG652769
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2034046..2045671

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ISF26_RS09935 (ISF26_09875)	-	2035902..2036387 (+)	486	WP_230843729.1	helix-turn-helix domain-containing protein	-
ISF26_RS09940 (ISF26_09880)	-	2036467..2037033 (+)	567	WP_230843730.1	PIN domain-containing protein	-
ISF26_RS09945 (ISF26_09885)	rpaB	2037126..2037851 (-)	726	WP_041244642.1	response regulator transcription factor RpaB	-
ISF26_RS09950 (ISF26_09890)	-	2038114..2038878 (-)	765	WP_230843731.1	sigma-70 family RNA polymerase sigma factor	-
ISF26_RS09955 (ISF26_09895)	pilB/pilB1	2039046..2040671 (+)	1626	WP_230843732.1	GspE/PulE family protein	Machinery gene
ISF26_RS09960 (ISF26_09900)	-	2040697..2041854 (+)	1158	WP_230843733.1	type IV pilus twitching motility protein PilT	-
ISF26_RS09965 (ISF26_09905)	-	2041875..2043107 (+)	1233	WP_230843734.1	type II secretion system F family protein	-
ISF26_RS09970 (ISF26_09910)	-	2043309..2044463 (+)	1155	Protein_1976	IS3 family transposase	-
ISF26_RS09975 (ISF26_09915)	-	2044633..2045586 (+)	954	WP_230843735.1	S-layer homology domain-containing protein	-

Sequence

Protein

• Length: 541 a.a.
• Molecular weight: 58400.13 Da
• Isoelectric Point: 6.8115

>NTDB_id=497061 ISF26_RS09955 WP_230843732.1 2039046..2040671(+) (pilB/pilB1) [Gloeobacter morelensis MG652769]
MASAQRLFELKIRYGVAAFDPAGVDPVRLRRLVETWLTIESCAHHRLLPVREEEGALAVAMVDPERLEALDELQRRGRLR
GLALRRLAIDAQDFALLLEACQAAPMEPEAAAGELEAPVVRLSSQILDRAVAEGASDIHIEPQPAGVRVRLRRDGVLHEA
GEPLSAELGLALVSRFKILSELDIAERRLPQDGRLYRRVGERAVDFRVSTLPGRFGEKVVLRLLDNAATRLGLDQLIGEA
GTLTAVRALIRRPHGLFLVTGPTGSGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGITQVQVIREKGLDFARILRALL
RQDPDIVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIASALVGVLAQRLLRRLCSECKEA
YVPPPERLARFGLTGGTYFQVRGQRGAACPGCGGVGYKGRVGVYEIMSLGEHLRQLIGREATTQTLREAAAAGGMRSLLA
GSLDLARAGVTSLEEVERVTLCDTDNAASPSAASHPICRGCGGELHSEWLACPYCTLPRID

Nucleotide

• Length: 1626 bp

>NTDB_id=497061 ISF26_RS09955 WP_230843732.1 2039046..2040671(+) (pilB/pilB1) [Gloeobacter morelensis MG652769]
ATGGCATCCGCGCAGCGATTGTTCGAATTGAAGATCCGCTATGGCGTCGCCGCCTTCGACCCCGCCGGGGTCGATCCGGT
TCGCCTGCGGCGGTTGGTCGAGACCTGGCTAACTATCGAAAGTTGTGCCCACCACCGGCTGCTGCCGGTGCGCGAGGAGG
AGGGGGCGCTGGCTGTGGCGATGGTCGATCCTGAGCGCCTCGAAGCCCTCGACGAACTGCAGCGGCGGGGCCGGTTGCGC
GGCCTCGCCCTGCGCCGCCTCGCCATCGACGCCCAGGATTTTGCCTTGTTGCTTGAAGCCTGCCAGGCAGCGCCGATGGA
GCCGGAGGCAGCGGCGGGCGAACTGGAGGCGCCCGTGGTGCGGCTTTCCTCCCAGATTCTCGATCGGGCGGTAGCGGAAG
GGGCCTCCGACATCCACATCGAACCGCAGCCCGCCGGGGTGCGCGTCCGCCTGCGCCGGGACGGCGTGCTGCACGAGGCC
GGCGAACCGCTGAGCGCCGAGCTGGGATTGGCGCTGGTGTCGCGCTTCAAGATCCTCTCGGAACTGGATATTGCCGAGCG
CCGTCTCCCCCAGGACGGCCGTTTGTACCGCCGCGTCGGGGAGCGGGCCGTCGATTTTCGCGTCTCGACGTTGCCGGGGC
GCTTCGGAGAGAAAGTGGTGCTCAGGCTGCTCGACAACGCCGCCACCCGCCTGGGGCTCGACCAGCTGATTGGCGAGGCC
GGGACGCTCACGGCGGTGCGCGCATTGATTCGTCGCCCCCACGGGTTGTTTCTGGTCACCGGTCCCACCGGCTCGGGCAA
GACGACCACCCTCTACGCGGCGCTTGCCGAGCGCAACGAGCCGGGGGTCAACATCGCCACCGCCGAAGATCCGGTGGAGT
ACACCCTGGCCGGGATCACCCAGGTGCAGGTGATCCGCGAGAAGGGCCTCGATTTTGCCCGCATTTTGCGCGCGCTGCTC
CGGCAAGATCCCGATATCGTGCTGGTGGGGGAGACCCGCGACCGCGAGACCGCCCAGACCGCCATCGAAGCGGCCCTCAC
CGGCCACCTGGTGCTCACCAGCCTGCACACCAACGACGCGGCCGGGGCGGTTGCGCGCCTCAGGGAGCTGGGGGTGGCCC
CCTATTTGATCGCCAGTGCCCTGGTGGGCGTGCTCGCCCAGCGGCTGTTGCGCCGGCTTTGCAGCGAGTGCAAAGAGGCG
TATGTACCGCCTCCTGAGCGCCTCGCCCGCTTTGGCCTCACAGGCGGGACGTACTTTCAGGTGCGGGGGCAGAGGGGAGC
AGCCTGCCCCGGCTGCGGCGGCGTAGGTTATAAAGGGCGCGTCGGAGTTTACGAAATCATGTCCCTGGGAGAACACCTGC
GCCAACTGATCGGCCGCGAAGCCACCACCCAGACGCTGCGCGAGGCGGCGGCGGCGGGCGGGATGCGCTCGCTGCTGGCA
GGTTCTCTGGATCTGGCCCGCGCGGGGGTAACCAGCTTAGAGGAAGTCGAGCGCGTCACCCTCTGCGACACTGACAACGC
GGCAAGCCCCTCGGCCGCGAGCCACCCCATCTGCCGCGGCTGCGGCGGCGAGTTGCACAGCGAATGGCTCGCCTGTCCCT
ACTGCACTCTGCCCCGGATCGATTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (4-105)

• Type II/IV secretion system protein (T2SSE) (126-392)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB/pilB1	Synechocystis sp. PCC 6803	50.083	100	0.555
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.767	92.052	0.384
pilB	Vibrio parahaemolyticus RIMD 2210633	42.127	90.388	0.381
pilF	Thermus thermophilus HB27	42.083	88.725	0.373
pilB	Vibrio campbellii strain DS40M4	41.875	88.725	0.372
pilB	Acinetobacter baylyi ADP1	39.053	93.715	0.366
pilB	Legionella pneumophila strain ERS1305867	37.595	96.858	0.364