Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   INT78_RS10615 Genome accession   NZ_CP063774
Coordinates   2227714..2228838 (-) Length   374 a.a.
NCBI ID   WP_000228517.1    Uniprot ID   E2QFE2
Organism   Escherichia coli O25b:H4-ST131     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2225298..2234796 2227714..2228838 within 0


Gene organization within MGE regions


Location: 2225298..2234796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INT78_RS10595 (INT78_10600) aroE 2225298..2226116 (-) 819 WP_000451255.1 shikimate dehydrogenase -
  INT78_RS10600 (INT78_10605) tsaC 2226121..2226693 (-) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  INT78_RS10605 (INT78_10610) yrdD 2226698..2227240 (-) 543 WP_001296461.1 type I DNA topoisomerase -
  INT78_RS10610 (INT78_10615) smg 2227269..2227742 (-) 474 WP_000460673.1 DUF494 family protein Smg -
  INT78_RS10615 (INT78_10620) dprA 2227714..2228838 (-) 1125 WP_000228517.1 DNA-protecting protein DprA Machinery gene
  INT78_RS10620 (INT78_10625) def 2228968..2229477 (+) 510 WP_000114984.1 peptide deformylase -
  INT78_RS10625 (INT78_10630) fmt 2229492..2230439 (+) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  INT78_RS10630 (INT78_10635) rsmB 2230491..2231780 (+) 1290 WP_000744765.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  INT78_RS10635 (INT78_10640) trkA 2231802..2233178 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  INT78_RS10640 (INT78_10645) mscL 2233308..2233721 (+) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  INT78_RS10645 (INT78_10650) arfA 2233718..2233936 (-) 219 WP_000092695.1 alternative ribosome-rescue factor ArfA -
  INT78_RS10650 (INT78_10655) zntR 2233992..2234417 (-) 426 WP_000285610.1 Zn(2+)-responsive transcriptional regulator -
  INT78_RS10655 (INT78_10660) yhdN 2234428..2234796 (-) 369 WP_000266504.1 DUF1992 domain-containing protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40910.86 Da        Isoelectric Point: 6.0395

>NTDB_id=496743 INT78_RS10615 WP_000228517.1 2227714..2228838(-) (dprA) [Escherichia coli O25b:H4-ST131]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=496743 INT78_RS10615 WP_000228517.1 2227714..2228838(-) (dprA) [Escherichia coli O25b:H4-ST131]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39