Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   INQ40_RS09900 Genome accession   NZ_CP063772
Coordinates   2171140..2172294 (-) Length   384 a.a.
NCBI ID   WP_193984178.1    Uniprot ID   A0A7S6UEY3
Organism   Lysobacter sp. H21R4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2166140..2177294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ40_RS09875 (INQ40_09875) - 2167435..2168385 (-) 951 WP_194340294.1 aspartate carbamoyltransferase catalytic subunit -
  INQ40_RS09880 (INQ40_09880) ruvX 2168478..2168951 (-) 474 Protein_1923 Holliday junction resolvase RuvX -
  INQ40_RS09885 (INQ40_09885) - 2168948..2169514 (-) 567 WP_194340296.1 YqgE/AlgH family protein -
  INQ40_RS09890 (INQ40_09890) - 2169517..2170134 (+) 618 WP_194340297.1 DNA-3-methyladenine glycosylase I -
  INQ40_RS09895 (INQ40_09895) - 2170171..2170998 (+) 828 WP_194340298.1 META and DUF4377 domain-containing protein -
  INQ40_RS09900 (INQ40_09900) pilU 2171140..2172294 (-) 1155 WP_193984178.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ40_RS09905 (INQ40_09905) pilT 2172353..2173390 (-) 1038 WP_043957729.1 type IV pilus twitching motility protein PilT Machinery gene
  INQ40_RS09910 (INQ40_09910) proC 2173694..2174557 (+) 864 WP_194340299.1 pyrroline-5-carboxylate reductase -
  INQ40_RS09915 (INQ40_09915) - 2174601..2175077 (+) 477 WP_194340300.1 DUF4426 domain-containing protein -
  INQ40_RS09920 (INQ40_09920) - 2175089..2175883 (+) 795 WP_194342512.1 cobalamin-binding protein -
  INQ40_RS09925 (INQ40_09925) pdxH 2175943..2176545 (-) 603 WP_194340301.1 pyridoxamine 5'-phosphate oxidase -
  INQ40_RS09930 (INQ40_09930) - 2176606..2176809 (-) 204 WP_193984187.1 dodecin family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42850.21 Da        Isoelectric Point: 6.4191

>NTDB_id=496698 INQ40_RS09900 WP_193984178.1 2171140..2172294(-) (pilU) [Lysobacter sp. H21R4]
MNTTQSTNETIDFTSFLKLMAHQKASDLFITAGMPPSMKVNGVLQPITKSPLTPQQSRDLVLNVMNPAQREEFEKTHEAN
FAIGLANVGRFRVSCFYQRNQVGMVLRRIETRIPTIDELNLPPVLKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQN
STGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHA
NNANQAMDRMINFFPEDRRNQLLMDLSLNLKGVIAQQLIPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKEST
QLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGNAQTLAAGMDGVEVAEIRD

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=496698 INQ40_RS09900 WP_193984178.1 2171140..2172294(-) (pilU) [Lysobacter sp. H21R4]
ATGAACACGACCCAGAGCACCAACGAGACGATCGACTTCACGTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCCTCCGA
CCTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTCAACGGCGTCCTGCAGCCGATCACCAAGAGCCCGCTGACGC
CGCAGCAAAGCCGCGACCTGGTGCTCAACGTGATGAATCCGGCCCAACGCGAGGAGTTCGAGAAGACCCACGAGGCGAAC
TTCGCGATCGGGCTGGCCAACGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCG
CCGGATCGAGACCCGGATTCCGACCATCGACGAGCTGAACCTGCCGCCGGTGCTGAAGACCCTGGCGATGACCAAGCGCG
GGATCATCCTGTTCGTCGGCGCCACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAAC
TCGACCGGCCACATCATCACCATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATTACCCAGCGCGA
GGTCGGCATCGACACCGACAGCTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCATGATCGGTG
AGGTGCGCACCCGCGAGGGCATGGACCACGCGATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACGCTGCACGCC
AACAACGCCAACCAGGCGATGGACCGGATGATCAACTTCTTCCCCGAGGACCGGCGCAACCAGTTGCTGATGGACCTGTC
GCTGAACCTGAAGGGCGTGATCGCCCAGCAGCTGATCCCGACCCCGGACGGCAAGGCGCGCCGGGTCGCGATGGAGATCC
TGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACC
CAGCTGGGCATGAAGACCTTCGACCAGGCGCTTTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCCTTGCG
CTACGCCGACTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTTGCGCAGGGCGGCAACGCGCAGACCCTCGCCGCGGGCA
TGGACGGCGTGGAAGTGGCCGAGATCCGCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6UEY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

95.573

0.646
  pilU Acinetobacter baylyi ADP1

64.706

92.969

0.602
  pilU Vibrio cholerae strain A1552

54.775

92.708

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.74

95.052

0.406