Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF732_RS02355 Genome accession   NZ_CP063745
Coordinates   443813..444310 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain NAS_AN_212     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438813..449310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF732_RS02315 (IF732_02315) - 438858..439844 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF732_RS02320 (IF732_02320) - 439844..440173 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF732_RS02325 (IF732_02325) - 440170..440793 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF732_RS02330 (IF732_02330) comGA 440845..441819 (+) 975 WP_031872164.1 competence type IV pilus ATPase ComGA Machinery gene
  IF732_RS02335 (IF732_02335) comGB 441791..442861 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF732_RS02340 (IF732_02340) comGC 442919..443185 (+) 267 WP_000927911.1 competence type IV pilus major pilin ComGC Machinery gene
  IF732_RS02345 (IF732_02345) comGD 443163..443609 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF732_RS02350 (IF732_02350) comGE 443596..443895 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF732_RS02355 (IF732_02355) comGF 443813..444310 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF732_RS02360 (IF732_02360) - 444407..444553 (+) 147 WP_001789879.1 hypothetical protein -
  IF732_RS02365 (IF732_02365) - 444543..445067 (+) 525 WP_001015120.1 shikimate kinase -
  IF732_RS02370 (IF732_02370) gcvT 445226..446317 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF732_RS02375 (IF732_02375) gcvPA 446337..447683 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF732_RS02380 (IF732_02380) gcvPB 447676..449148 (+) 1473 WP_000202184.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=496424 IF732_RS02355 WP_001825340.1 443813..444310(+) (comGF) [Staphylococcus aureus strain NAS_AN_212]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=496424 IF732_RS02355 WP_001825340.1 443813..444310(+) (comGF) [Staphylococcus aureus strain NAS_AN_212]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97