Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IF732_RS02330 Genome accession   NZ_CP063745
Coordinates   440845..441819 (+) Length   324 a.a.
NCBI ID   WP_031872164.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_212     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435845..446819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF732_RS02295 (IF732_02295) rpmG 436305..436454 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  IF732_RS02300 (IF732_02300) - 436663..437202 (+) 540 WP_000164554.1 5-formyltetrahydrofolate cyclo-ligase -
  IF732_RS02305 (IF732_02305) - 437214..438677 (+) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  IF732_RS02310 (IF732_02310) - 438658..438861 (+) 204 WP_000087559.1 YqgQ family protein -
  IF732_RS02315 (IF732_02315) - 438858..439844 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF732_RS02320 (IF732_02320) - 439844..440173 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF732_RS02325 (IF732_02325) - 440170..440793 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF732_RS02330 (IF732_02330) comGA 440845..441819 (+) 975 WP_031872164.1 competence type IV pilus ATPase ComGA Machinery gene
  IF732_RS02335 (IF732_02335) comGB 441791..442861 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF732_RS02340 (IF732_02340) comGC 442919..443185 (+) 267 WP_000927911.1 competence type IV pilus major pilin ComGC Machinery gene
  IF732_RS02345 (IF732_02345) comGD 443163..443609 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF732_RS02350 (IF732_02350) comGE 443596..443895 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF732_RS02355 (IF732_02355) comGF 443813..444310 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF732_RS02360 (IF732_02360) - 444407..444553 (+) 147 WP_001789879.1 hypothetical protein -
  IF732_RS02365 (IF732_02365) - 444543..445067 (+) 525 WP_001015120.1 shikimate kinase -
  IF732_RS02370 (IF732_02370) gcvT 445226..446317 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36867.93 Da        Isoelectric Point: 8.8516

>NTDB_id=496419 IF732_RS02330 WP_031872164.1 440845..441819(+) (comGA) [Staphylococcus aureus strain NAS_AN_212]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTAIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=496419 IF732_RS02330 WP_031872164.1 440845..441819(+) (comGA) [Staphylococcus aureus strain NAS_AN_212]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTACGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TGCTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997